Periodic acid Schiff technique detail explanation
Q: Write the materials, reagents, Principle and procedure for the Periodic acid Schiff technique
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Q: Write the protocol for the periodic acid schiff technique
A:
Q: Citrate test What critical enzyme allows the organism to acquire carbon from the medium?
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Q: Technique Initial IMAC IEX Gel Filtration Protein (mg) 3467.8 196.7 153.6 142.1 Enzyme activity…
A: Total protein is the the amount of protein in the mixture. Enzyme activity is the μmol of product…
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Q: briefly explain the principle behind this technique; gel-filtration chromatography
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Q: When preparing samples for SDS-PAGE, what is the purpose of boiling the samples at 95°C?
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Q: Citrate test What color indicates a rise in pH from the production of alkaline or basic products?
A: Citrate or citrate utilization test is one of the biochemical tests (IMViC tests) that are used for…
Q: Illustrate the dilution series used and label the final dilution of each dilution.
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Q: Citrate test What is the only souce of carbon in this medium?
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Q: Citrate test What pH idicateor is in this medium?
A: It is required to identify the pH indicator in citrate test.
Periodic acid Schiff technique detail explanation
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- When preparing samples for SDS-PAGE, what is the purpose of boiling the samples at 95°C?How would you make two-fold serial dilutions such that the last tube is a 1:32 dilution of the original, concentrated material? Assume that you need to have at least 500 µl of each dilution, and you should be able to perform the dilutions in microfuge tubes with a maximum capacity of 1.5 ml.FOLIN-WU METHOD a. What is the type of blood sample used and how much of it is added to distilled water? b. What are the protein precipitants used and indicate the specific concentration of each? c. What is the color of the precipitate obtained? d. If the desired color of the mixture(blood sample and reagent mixture) is not achieved, what chemical is added drop by drop to obtain the desired color? Specify the conc.
- Which stationary phase is better in separation of the components of moringa extract for column chromatography? Sodium Bicarbonate or Silica Gel? And Explain.Why did the blank used in experimental procedures contain substrate, buffer and water?Methyl Red test What color is the ph indicator in the dropper bottle?
- briefly explain the principle behind this technique; gel-filtration chromatographyTopic: Isolation of Crude Ovalbumin from Egg White by Ammonium Sulfate Precipitation (Salting Out) The computed amount of powdered ammonium sulfate was added to the egg white sample portion byportion with constant stirring while submerged in an ice bath. The solution is expected to become moreturbid, and a white precipitate is expected to form. The resulting mixture was then filtered using a cheesecloth. The residue was discarded, and 30.0 mL of thefiltrate was brought from 40% to 60% saturation by adding the required amount of powdered ammoniumsulfate in the same manner as the previous addition. After adding ammonium sulfate, the mixture was allowed to stand with occasional stirring for 30 minutes inan ice bath. The formation of a white precipitate is expected to happen QUESTION: Explain the significance of allowing the mixture to be submerged in an ice bath for a given time.Citrate test What color indicates a rise in pH from the production of alkaline or basic products?