One of the most common buffers used in protein chemistry is a weak base called TRIS (the nitrogen atom of the amino group is the basic portion of the molecule): Но. -NH2 (HOCH2);CNH2 = TRIS HO A biochemist prepares a buffer solution by adding enough TRIS and 12 M HCI to give 1.0 L of a buffer solution that has concentrations [TRIS] = 0.34 M and [TRISH*] = 0.71 M. (pKµ of TRIS is 5.91.) (a) Calculate the pH of the buffer solution.

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- /1 E
One of the most common buffers used in protein chemistry is a weak base called TRIS (the nitrogen atom of the amino group is the
basic portion of the molecule):
HO.
-NH2
(HOCH,),CNH,
TRIS
HO
HO.
A biochemist prepares a buffer solution by adding enough TRIS and 12 M HCI to give 1.0 L of a buffer solution that has concentrations
[TRIS] = 0.34 M and [TRISH*] = 0.71 M. (pKp of TRIS is 5.91.)
(a) Calculate the pH of the buffer solution.
pH = i
(b) Suppose that 9.3 mL of 12 M HCl is added to 12 L of the buffer solution; calculate the new pH of the solution.
pH = i
%D
Transcribed Image Text:Question 6 of 25 - /1 E One of the most common buffers used in protein chemistry is a weak base called TRIS (the nitrogen atom of the amino group is the basic portion of the molecule): HO. -NH2 (HOCH,),CNH, TRIS HO HO. A biochemist prepares a buffer solution by adding enough TRIS and 12 M HCI to give 1.0 L of a buffer solution that has concentrations [TRIS] = 0.34 M and [TRISH*] = 0.71 M. (pKp of TRIS is 5.91.) (a) Calculate the pH of the buffer solution. pH = i (b) Suppose that 9.3 mL of 12 M HCl is added to 12 L of the buffer solution; calculate the new pH of the solution. pH = i %D
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