For humans to maintain the pH of their blood close to 7.4, the body uses three buffer systems: carbonate, phosphate and proteins. If the pH of the blood goes below 6.8 or above 7.8, death may result. Imagine you want to make a phosphate buffer in the lab that resembles the bloods phosphate buffer. You start by mixing 0.95 M NaH2PO4 and 0.68 M Na2HPO4. H2PO4- + H2O ⇌ HPO42- + H+ pKa= 7.21 (5 marks total) a. From the reaction above indicate the weak acid and the conjugate base involved in making this buffer. (2 marks) b. Calculate the pH of the phosphate buffer solution. Provide your answer with 2 decimal places (2 marks) c. If the pH calculated in b. was the blood pH of an actual patient, would you say this patient’s blood pH is in the normal range or are they suffering from alkalosis or acidosis? (1 mark)
For humans to maintain the pH of their blood close to 7.4, the body uses three buffer systems: carbonate, phosphate and proteins. If the pH of the blood goes below 6.8 or above 7.8, death may result.
Imagine you want to make a phosphate buffer in the lab that resembles the bloods phosphate buffer. You start by mixing 0.95 M NaH2PO4 and 0.68 M Na2HPO4.
H2PO4- + H2O ⇌ HPO42- + H+ pKa= 7.21 (5 marks total)
a. From the reaction above indicate the weak acid and the conjugate base involved in making this buffer. (2 marks)
b. Calculate the pH of the phosphate buffer solution. Provide your answer with 2 decimal places (2 marks)
c. If the pH calculated in b. was the blood pH of an actual patient, would you say this patient’s blood pH is in the normal range or are they suffering from alkalosis or acidosis? (1 mark)
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