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Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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3. An enzyme is found to have an active site cysteine residue that plays a critical role in catalysis. At what pH does this cysteine operate efficiently in nucleophilic catalysis? Describe how proteins alter the pK of this side chain from its normal value in solution. 4. If you had a protein X, which is a soluble enzyme found inside the peroxisome, and you wished to separate it from a similar protein Y, which is an enzyme found embedded in the mitochondrial membrane, what would be your initial techniques for isolating these proteins?
Transcribed Image Text:3. An enzyme is found to have an active site cysteine residue that plays a critical role in catalysis. At what pH does this cysteine operate efficiently in nucleophilic catalysis? Describe how proteins alter the pK of this side chain from its normal value in solution. 4. If you had a protein X, which is a soluble enzyme found inside the peroxisome, and you wished to separate it from a similar protein Y, which is an enzyme found embedded in the mitochondrial membrane, what would be your initial techniques for isolating these proteins?
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