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ntrolling the cell cycle. Earlier researchers ha frog eggs (Xenopus) contained all the necess ired for DNA replication. This included protei noting factor (MPF). At the time of this study, ity and the research group wanted to test usi 1. In Figure 1 (a) MPF activity was tested for it ne (H1 in snerm chro matin ove hromatin ortain p

ntrolling the cell cycle. Earlier researchers ha frog eggs (Xenopus) contained all the necess ired for DNA replication. This included protei noting factor (MPF). At the time of this study, ity and the research group wanted to test usi 1. In Figure 1 (a) MPF activity was tested for it ne (H1 in snerm chro matin ove hromatin ortain p

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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In the following study, the investigators wanted to determine the role of cyclin B in controlling the cell cycle. Earlier researchers had found that extracts made from frog eggs (Xenopus) contained all the necessary proteins and machinery required for DNA replication. This included proteins that regulated the mitosis promoting factor (MPF). At the time of this study, cyclin B was show to affect MPF activity and the research group wanted to test using Xenopus egg extract in an assay. In Figure 1 (a) MPF activity was tested for its ability to phosphorylate Histone (H1) in sperm chromatin over a certain period of time. Additionally, the cyclin B concentration in the extract was measured. In figure 1b, the extract was tested after treatment with RNase which degraded only the mRNA and not RNA or FRNA in the extract. Knowing that cyclin B is a short-lived protein, why do you suppose the graph shows the results you see in figure 1b?
Transcribed Image Text:In the following study, the investigators wanted to determine the role of cyclin B in controlling the cell cycle. Earlier researchers had found that extracts made from frog eggs (Xenopus) contained all the necessary proteins and machinery required for DNA replication. This included proteins that regulated the mitosis promoting factor (MPF). At the time of this study, cyclin B was show to affect MPF activity and the research group wanted to test using Xenopus egg extract in an assay. In Figure 1 (a) MPF activity was tested for its ability to phosphorylate Histone (H1) in sperm chromatin over a certain period of time. Additionally, the cyclin B concentration in the extract was measured. In figure 1b, the extract was tested after treatment with RNase which degraded only the mRNA and not RNA or FRNA in the extract. Knowing that cyclin B is a short-lived protein, why do you suppose the graph shows the results you see in figure 1b?
Untreated extract (b) RNase-treated axtract Mitotic events -- MPF activity - Cyclin B cone Sperm chromatin Time Time > RNase-treated extract + wild-type cyclin B MRNA (d) RNase-treated extract + nondegradable cyclin B MRNA Mitotic events Sperm chromatin Time Mitotic arrest Time > IGURE 13-7 Experimental demonstration that the thesis and degradation of cyclin B are required for the ing of MPF activity and mitotic events in Xenopus egg acts. In all cases, MPF activity and cyclin B concentration a determined at various times after addition of sperm matin to an extract treated as indicatod. Microscopic vations detorminod the occurrence of early mitotic events (blue shading), including chromosome condensation and nuclear envelope breakdown, and of late events (orange shading). including chromosome decondensation and nuclear envelope reformation. Soe text for discussion. ISee A. W. Murray et al, 1989, Nature 339:275; adapted from A. Murray and T, Hunt, 1993, The Cel Cycle: An introduction, W. H. Freeman and Company)
Transcribed Image Text:Untreated extract (b) RNase-treated axtract Mitotic events -- MPF activity - Cyclin B cone Sperm chromatin Time Time > RNase-treated extract + wild-type cyclin B MRNA (d) RNase-treated extract + nondegradable cyclin B MRNA Mitotic events Sperm chromatin Time Mitotic arrest Time > IGURE 13-7 Experimental demonstration that the thesis and degradation of cyclin B are required for the ing of MPF activity and mitotic events in Xenopus egg acts. In all cases, MPF activity and cyclin B concentration a determined at various times after addition of sperm matin to an extract treated as indicatod. Microscopic vations detorminod the occurrence of early mitotic events (blue shading), including chromosome condensation and nuclear envelope breakdown, and of late events (orange shading). including chromosome decondensation and nuclear envelope reformation. Soe text for discussion. ISee A. W. Murray et al, 1989, Nature 339:275; adapted from A. Murray and T, Hunt, 1993, The Cel Cycle: An introduction, W. H. Freeman and Company)
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