Microscope You should always position the cells or tissues in the center of view before increasing magnification, because you know that the image will move right as you move the slide to the left. you know that the image will move up as you move the slide down you know that the center of field of view will be unchanged this is the best way to avoid breaking the slide with an objective

Microscope You should always position the cells or tissues in the center of view before increasing magnification, because you know that the image will move right as you move the slide to the left. you know that the image will move up as you move the slide down you know that the center of field of view will be unchanged this is the best way to avoid breaking the slide with an objective

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter15: Instrumentation In Medical Imaging

Section: Chapter Questions

Problem 9P

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PRELAB: Calculating Magnification Each lens makes the object look larger by a specified amount. To determine the total magnification, multiply the magnification of the eyepiece and the objective being used. Table 1: Microscope Magnification Magnifying power Objective Objective Eyepiece Total name Scanner 4 X Low power 100 X High power 40 χ PRELAB: Estimating the Size of an Object using a Microscope You will not be able to use a ruler to directly measure the size of a cell. Instead, you will use an indirect method by comparing the cell to the size of something you already know. The diameter of the microscope's field of view as seen through the eyepiece is a convenient length to use. A very small unit of measurement, the micron (µm) is used for measuring microscopic objects. The conversions are: 1 um = 0.001 mm 1 mm = 1000 µm Table 2: Field of View Diameters Objective Diameter name millimeter (mm) micron (um) Scanner 4 Low power High power 0.5 To estimate the size of an object, divide…
You should always position the cells or tissues in the center of view before increasing magnification, because... You know that the image will move right as you move the slide to the left. You know that the image will move up as you move the slide down. You know that the center of the field of view will be unchanged. This is the best way to avoid breaking the slide with an objective
Determine the total magnification of a microscope when using the 4X objective lens. (Showyour work/math)
PART C: CALCULATING THE DIAMETER OF THE FIELD OF VIEW (FOV)_ The field of view (FOV) is the circular area you can see when you look through the microscope. The diameter of the field of view is different depending on which objective lens you are using. For example, you are using the medium-power objective lens, then the area you can see is actually smaller than if you were using the low-power objective lens. Knowing The diameter of the field of view can help you estimate actual size of objects / cells seen through the microscope. When the revolving nose piece is turned to the low power objective lens, a dear plastic ruler can be placed on the microscope stage (see figure 1). Then, the coarse adjustment knob can be used to focus on the millimeter marks of the ruler making sure that one of the milimeter marks is at the left edge of the field of view (see figure 2). NOTE: Slage cip I-1000 Objects in the FOV are usually measured in micrometers (um). To convert, a FOV in mm, times it by 1000…
Differentiate between the limit of resolution of the typical light microscope and that of the unaided human eyes the relationship between the depth of field and magnification is that When magnification increases, the depth of field decreases. Name two procedures (steps) you should do to achieve the maximum resolution when using the oil immersion lens State the relationship between the working distance of an objective lens and its magnification power “The coarse focus knob can be used to adjust the focus when using any of the objective lenses”. Is this statement true or false? If it is false, please correct it. When using the oil immersion lens but you cannot focus, what should you should not do? And what you should do? What does this statement mean: “Your microscope is parfocal”? What is the significance of condenser lens? Multiple choice question: The most useful adjustment for increasing image contrast in low power magnification is _______ Closing down the diaphragm Closing one…
Which of the following is true about the relationship between depth of field and magnification? There is no relationship. When magnification increases, depth of field decreases. When magnification increases, depth of field increases. Stereomicroscopes offer a lesser depth of field than compound microscopes. The depth of field of a microscope determines how much magnification it can achieve.
These cells are being viewed under high power. Use the length of just one cell to estimate the number of cells that can fit into the FOV. Scanning power objective = 5X; Low power objective = 40X; High power objective = 100X; Eyepiece = 10X; Low power field of view (FOV) = 1.5 mm
Match the part of the microscope with its use. Dragged and dropped options on the right-hand side will be automatically saved. For keyboard navigation... SHOW MORE Stage Nosepiece Vertical Stage Knobs Course and Fine Adjustment Knobs Iris Diaphram III ||| Allows for changing objective lenses Move the stage side to side Hold the slide in place Control light passing through the stage Move the stage up and down
You have a microscope equipped with 10 x oculars and 4 objectives, which exhibit the following characteristics (M, NA): Tick the right answers:o Objectives B and C are equivalent.o Objective D will allow more detailed observation than objective C.o To see small structures, you should choose objective D rather than C.o Objective D has a lower resolution than objective C.o To observe large objects, it is better to use objective B rather than objective C
which objective lens is in place if the object you are viewing is magnified 1000x? (assume ocular lens magnification = 10x)
In light microscopy, when magnification is increased, the lens focuses closer to the sample. This makes the user more likely to ram the sample into the lens. Why is it common for the lens to focus closer to the sample at higher magnifications? What can be done to mitigate this risk?
Why are stereomicroscopes often referred to as dissecting microscopes? Stereomicroscopes come apart (dissect) easily. The large distance between the lens and the specimen makes them ideal for dissections. They are good for viewing much smaller objects. Only a few specimens will fit under the stereomicroscope. The two eyepiece lenses on a stereomicroscope give a better perception of depth.