Microbial products can be classified as three major categories. With the use of sketch, briefly explain the kinetic patterns of growth and product formation in batch fermentation.
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- Microbial fermentation can be carried out as either a once off ----------------- culture or a-------------------------- culture. The correct terminology for these respective cultures & the vessel the latter is carried out in are? Select one: a. continuous, batch, fermenter b. batch, continuous, chemostat c. batch, continuous, turbidostat d. batch, continuous, turbidostat e. continuous, batch, chemostatDescribe the characteristics of microorganisms must have to be useful in the industrial fermentation process. Explain why these characteristics are important.What is the key difference between reactions that result in the final product of fermentation to form lactic acid or alcohol? What significance do these differences have in terms of microbial technology?
- Provide a photograph of an autoclave with labels. Discuss briefly how autoclaving control microbial growth.What is the importance of fermentation to microbial metabolism? Please include citation.Menaquinones is produced by Flavobacterium meningosepticum via submerged fermentation with 60% working volume in 30-L fermentation. To obtain the crude menaquinones, the fermentation medium is centrifuged at 12,000 rpm for 15 minutes. The vacuum-freeze dried particles are crushed and collected. After that the samples are treated with methanol for 20 minutes. To remove polar lipid compounds from bacterial cells, HZ816 macroporous adsorption resin column is used with methanol-dichloromethane (l:1) as mobile phase and flow rate of 0.75 mL/min. Rotary evaporation at 500 C is carried out to concentrate the eluent. After that, the concentrated sample is purified with Bio-BeadsTM S-X3 to remove components without UV absorption. To obtain the homolog purified menaquinones, RP-C18 column is used. Recrystallization is carried out to obtain pure crystalized menaquinones. Answer the following: Indicate how to obtain the sample in the methanol solvent. Three different chromatography are used in…
- Write down the criteria for media selection and the basic requirements for media formulation for fermentation processes.Describe the industrial fermentation process of Cephalosporin by including the type of fermentation porcess (batch, fed-batch or Continous) and bioreactor used. Also describe how the media was prepared.Assume you are the lead industrial microbiologist in a company that produces cholesterol-lowering fungal metabolite such as lovastatin. Your team isolated a novel fungus that can be used for large-scale production of lovastatin. Briefly describe how you would produce 20 L of this product from a potato dextrose agar plate culture using a 20 L fermentor.
- Mannitol salt agar contains salt to prevent the growth of most microbes and mannitol plus a pH indicator to detect mannitol fermentation. Which component of mannitol salt agar makes it a selective medium?Explain how the microbe in your selected food undergoes fermentation biochemically speaking, and how does this impacts the food quality? The example I chose for the fermented food is Yogurt.Describe the industrial fermentation process of Penicillin and Cephalosporin.Include points: -name of strain used as inoculum-type of fermentation process (batch/fed-batch/continuous) and bioreactor (airlift/ continous stirred Tank/ any other) used-media preparation (carbon source, nitrogen source, salts, oxygen requirement)-pH and remperature maintained