Methods of Protein denaturation: Experimental Procedures of Salts of Heavy Metals, Alkaloidal Reagents and Salting out
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Methods of Protein denaturation:
Experimental Procedures of Salts of Heavy Metals, Alkaloidal Reagents and Salting out
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- Complete the table below regarding the different laboratory tests done for Amino Acids and Proteins Tests Procedure (Chemical Reagents Added) Positive Results/ Observations (ex: color of solution, precipitate formation, etc) Positive for (provide details like functional group responsible) Molisch Test Benedict’s Test Barfoed’s Test Moore’s Test Bial’s Test Seliwanoff’s Test Osazone Test Fehling’s Test Tollen’s Test Mucic Acid Test Lugol’s Test/ Iodine Test Hydrolysis w/ Benedict’s Test Hydrolysis w/ Iodine Test Provide the expected results with the samples given below: For negative results just leave it blank, for positive results write “(+) then the expected observation” ex: + blue solution Samples Molisch Test Benedict’s Test Barfoed’s Test Moore’s Test Bial’s Test Seliwanoff’s Test Glucose Galactose Fructose Ribose…SDS-PAGE reagents that play a role in denaturing the protein sample include (Select all that applies) Bromophenol blue APS Sodium Dodecyl Sulfate Acetic Acid Glycerol Heat Beta-Mercaptoethanol TEMEDIn protein electrophoresis, what reagent present in the sample buffer is used to eliminate differences in the charge densities of proteins? Tris-HCL ph 8.8 2-mecraptothanol SDS(sodium dodecyl sulfate) Tris-HCL PH 6.8
- Example of a Protein Purification Scheme: Purification of the Enzyme Xanthine Dehydrogenase from a Fungus Volume Total Total Specific Percent Fraction (mL) Protein (mg) Activity Activity Recovery 1. Crude extract 2. Salt precipitate 3. Ion-exchange chromatography |4. Molecular-sieve chromatography 5. Immunoaffinity chromatography 3,800 22,800 2,460 0.108 100 165 2,800 1,190 0.425 48 65 100 720 7.2 29 40 14.5 23 1.8 275 152.108 11 Calculate the specific activity of step#4. Note that percent recovery=% Yield.Which of the following techniques can best separate a protein from other proteins on the basis of size? Electrophoresis in polyacrylamide gels containing SDS lon-exchange chromatography Dialysis using a membrane . Isoelectric focusing Differential centrifugationSearch for the principles and the uses of the following methods for protein measurements and provide an image that will serve as your visual reference. Serum Protein Electrophoresis Isoelectric Focusing Ion Exchange Chromatography Turbidimetry Solvent Fractionation
- Why is it not advisable to use adhesive mixture if protein histological investigation are contemplatedWhich ane of the following reagents cannot be used for determination of lipid concentrations? Folch's reagent Bloors reagent Aniline reagent 03008 Phosphovanillin reagentChoose the correct description for the Biomolecule Test demonstrated in the figure. reagent త The polysaccharide starch is tested using Lugol's lodine The protein is tested using Biuret Reagent. The protein is tested using Lugol's lodine The polysaccharide starch is tested using Biuret Reagent O O O