What are the principles involved in the isolation and purification of proteins? Isolation Purification

functions of protein in a eukaryotic cell.

How an alpha helix and a beta pleated sheet differ from one another?

Ninhydrin Test Reagent’s Composition   Observations:   Inferences: What is the principle behind this test?     Can ninhydrin test distinguish amino acids and proteins? Can this test be used to detect the presence of proteins in a sample? Elaborate your answers.

if comparing proteins with a Tm=54 degrees C and one with a Tm =78 degrees c, which is more stable

Explain the importance of protein denaturation.

What physical method is most frequently use to determine three dimensional structures of protein? Fluorescence spectroscopy Electron microscopy X ray crystallography Nuclear magnetic resonance spectroscopy Mass spectrometry

Explain the contribution of features of the beta-sheet to the stability of the protein.

Protein denaturation results in a disruption of the

[Ten - Biomolecules] INSTRUCTIONS — Answer the following multiple-choice questions and EXPLAIN in 3-5 sentences why you chose that answer. — Answer properly Questions; Thomas was purifying an enzyme from a homogenate of muscle cells. He went through seven steps of purification and found that the enzyme activity was the same as the homogenate value. On the eighth step, when the protein washighly pure, the enzyme activity rose to five times that of the homogenate value. Can you suggest a possible reason? A. the homogenate assay was wrong B. step eight has co-purified an activator of the enzyme. C. the enzyme has an inhibitor present in the muscle cell homogenate D. the temperature at step eight was just right for the enzyme activity.