Metagenomics is aimed ata. determining the complete genome sequence of newlyidentified microorganisms.b. mapping the genes along chromosomes of newly identifiedmicroorganisms.c. determining the sequence of DNA fragments in environmentalsamples.d. determining the functions of all of the genes in a givenspecies’ genome.

Genetic information has become part of our culture and it is difficult to tell the difference between unmodified and genetically modified food sources such as plant and animals. After reading this module’s material regarding vectors in biotechnology, consider the potential for nanotechnology and scientific advancement.Research nanotechnology and its potential use in biotechnology. In one or two paragraphs, explain the potential advantages and disadvantages of nanotechnology in health care, agriculture, or industry and discuss whether you would or would not support further research.

Give three advantages ‘Next Gen sequencing’ has over Sanger sequencing for clinical microbiology.

Q. How can you design your RT-PCR experiment to control for gDNA contamination? A. Use forward and reverse primers that bind to the same exon.  B. Run a control lane where only RT was performed and not PCR. C. Run a control lane where mRNA has been amplified using PCR. D. Use forward and reverse primers that span the junction of 2 separate exons.

How has metagenomics improved the discovery ofnovel, useful products in biotechnology?

Phage Mu may be integrated into E. coli genomic DNA via Select one: O a. recombination catalyzed by homologous recombination enzymes. O b. conservative transposition O c. integration catalyzed by integrase. d. replicative transposition

Describe a biotechnology technique(s) that you could use to study how active (how much transcription is occurring) in one specific gene.

Sequencing a genome and identifying individual genes are processes typically carried out  - A. via manual transcription by a team of genetic scientists. B. by computers using high-throughput methods. C. by hand with a team of information scientists. D. using experimental processes.

A molecular marker is a                           found at a specific siteon a chromosome that has properties that allow it to be                                                          . a. colored dye, visualized via microscopyb. colored dye, visualized on a gelc. segment of DNA, uniquely identified using molecular toolsd. segment of DNA, visualized via microscopy