Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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Why would this data be controversial
MDCK cells
Jurkat cells
45
sphingolipidwGPL
cholesterouGPL
45
35
cholesterol GPL
2.5
25
1.5
15
0.5
05
Fig.
trea
Fig. 4. Lipid contents of DRMS from MDCK and Jurkat cells. Lipids from the
TLC plates shown in Fig. 3 were quantified and grouped into sphingolipids
(GalC + GluC+ LacC + SM + Gb, for MDCK cells, GluC+ SM for Jurkat cells,
intensities taken from "C-labeled lipids), cholesterol and glycerophospholip-
ids (PE + PI+ PS+ PC, intensities taken from "P-labeled lipids). Sphingolipid/
glycerophospholipid and cholesterol/glycerophospholipid ratios relative to
the ratios for TCMS are shown. GPL, glycerophospholipids.
with
cont
solu
sess
stre
of pe
for
ber of carbon atoms and/or the number of double bonds in the
fatty acid moicties, were detected. For cach species, its percent
fraction of the total PC was calculated. In addition, the relative
concentration of cach spccies was determined to allow compar-
ison of the amounts present in different DRMS (see Table 1,
which is published as supporting information on the PNAS web
site, www.pnas.org). PC spccies were then grouped according to
the degree of saturation of their fatty acids (Fig. 5).
In MDCK cells,-60% of the total cell membrane PC was
monounsaturated, <10% was fully saturated, and -30% pos-
unsa
dete
Int
of sa
with
noun
from
partit
Jurka
portic
domai
of gly
results
ferent
Take
MDCK cells
TCM
70
Tween 20
as qua
ent det
by the
60
Bry 50
Lubral WX
50
Brj 98
prepara
arbitrary units
TCM
Tween
Brij 58
Lubrol
Brij 98
Brij 96
Triton
CHAPS
TCM
Tween
Brij 58
Lubrol
Brij 98
otal PC
Brij 96
Triton
CHAPS
expand button
Transcribed Image Text:MDCK cells Jurkat cells 45 sphingolipidwGPL cholesterouGPL 45 35 cholesterol GPL 2.5 25 1.5 15 0.5 05 Fig. trea Fig. 4. Lipid contents of DRMS from MDCK and Jurkat cells. Lipids from the TLC plates shown in Fig. 3 were quantified and grouped into sphingolipids (GalC + GluC+ LacC + SM + Gb, for MDCK cells, GluC+ SM for Jurkat cells, intensities taken from "C-labeled lipids), cholesterol and glycerophospholip- ids (PE + PI+ PS+ PC, intensities taken from "P-labeled lipids). Sphingolipid/ glycerophospholipid and cholesterol/glycerophospholipid ratios relative to the ratios for TCMS are shown. GPL, glycerophospholipids. with cont solu sess stre of pe for ber of carbon atoms and/or the number of double bonds in the fatty acid moicties, were detected. For cach species, its percent fraction of the total PC was calculated. In addition, the relative concentration of cach spccies was determined to allow compar- ison of the amounts present in different DRMS (see Table 1, which is published as supporting information on the PNAS web site, www.pnas.org). PC spccies were then grouped according to the degree of saturation of their fatty acids (Fig. 5). In MDCK cells,-60% of the total cell membrane PC was monounsaturated, <10% was fully saturated, and -30% pos- unsa dete Int of sa with noun from partit Jurka portic domai of gly results ferent Take MDCK cells TCM 70 Tween 20 as qua ent det by the 60 Bry 50 Lubral WX 50 Brj 98 prepara arbitrary units TCM Tween Brij 58 Lubrol Brij 98 Brij 96 Triton CHAPS TCM Tween Brij 58 Lubrol Brij 98 otal PC Brij 96 Triton CHAPS
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