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MDCK cells Jurkat cells 45 sphingolipida/GPL 45 sphngolpds/GPL cholesterolGPL 4. 4. 3.5 cholesteroGPL 35 2.5 25 1.5 15 0.5 05 Fig. 4. Lipid contents of DRMS from MDCK and Jurkat cells. Lipids from the TLC plates shown in Fig. 3 were quantified and grouped into sphingolipids (GalC + GluC+ LacC+ SM + Gb, for MDCK cells, GluC+ SM for Jurkat cells, intensities taken from "C-labeled lipids), cholesterol and glycerophospholip- ids (PE+ PI+ PS + PC, intensities taken from "P-labeled lipids). Sphingolipid/ glycerophospholipid and cholesterol/glycerophospholipid ratios relative to the ratios for TCMS are shown. GPL, glycerophospholipids. arbitrary units TCM Tween Brij 58 Lubrol Brij 98 Brij 96 Triton CHAPS TCM Tween Brij 58 Lubrol Brij 98 Brij 96 Triton CHAPS

MDCK cells Jurkat cells 45 sphingolipida/GPL 45 sphngolpds/GPL cholesterolGPL 4. 4. 3.5 cholesteroGPL 35 2.5 25 1.5 15 0.5 05 Fig. 4. Lipid contents of DRMS from MDCK and Jurkat cells. Lipids from the TLC plates shown in Fig. 3 were quantified and grouped into sphingolipids (GalC + GluC+ LacC+ SM + Gb, for MDCK cells, GluC+ SM for Jurkat cells, intensities taken from "C-labeled lipids), cholesterol and glycerophospholip- ids (PE+ PI+ PS + PC, intensities taken from "P-labeled lipids). Sphingolipid/ glycerophospholipid and cholesterol/glycerophospholipid ratios relative to the ratios for TCMS are shown. GPL, glycerophospholipids. arbitrary units TCM Tween Brij 58 Lubrol Brij 98 Brij 96 Triton CHAPS TCM Tween Brij 58 Lubrol Brij 98 Brij 96 Triton CHAPS

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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MDCK cells Jurkat cells 45 sphingolipidwGPL cholesterouGPL 45 35 cholesterol GPL 2.5 25 1.5 15 0.5 05 Fig. trea Fig. 4. Lipid contents of DRMS from MDCK and Jurkat cells. Lipids from the TLC plates shown in Fig. 3 were quantified and grouped into sphingolipids (GalC + GluC+ LacC + SM + Gb, for MDCK cells, GluC+ SM for Jurkat cells, intensities taken from "C-labeled lipids), cholesterol and glycerophospholip- ids (PE + PI+ PS+ PC, intensities taken from "P-labeled lipids). Sphingolipid/ glycerophospholipid and cholesterol/glycerophospholipid ratios relative to the ratios for TCMS are shown. GPL, glycerophospholipids. with cont solu sess stre of pe for ber of carbon atoms and/or the number of double bonds in the fatty acid moicties, were detected. For cach species, its percent fraction of the total PC was calculated. In addition, the relative concentration of cach spccies was determined to allow compar- ison of the amounts present in different DRMS (see Table 1, which is published as supporting information on the PNAS web site, www.pnas.org). PC spccies were then grouped according to the degree of saturation of their fatty acids (Fig. 5). In MDCK cells,-60% of the total cell membrane PC was monounsaturated, <10% was fully saturated, and -30% pos- unsa dete Int of sa with noun from partit Jurka portic domai of gly results ferent Take MDCK cells TCM 70 Tween 20 as qua ent det by the 60 Bry 50 Lubral WX 50 Brj 98 prepara arbitrary units TCM Tween Brij 58 Lubrol Brij 98 Brij 96 Triton CHAPS TCM Tween Brij 58 Lubrol Brij 98 otal PC Brij 96 Triton CHAPS
Transcribed Image Text:MDCK cells Jurkat cells 45 sphingolipidwGPL cholesterouGPL 45 35 cholesterol GPL 2.5 25 1.5 15 0.5 05 Fig. trea Fig. 4. Lipid contents of DRMS from MDCK and Jurkat cells. Lipids from the TLC plates shown in Fig. 3 were quantified and grouped into sphingolipids (GalC + GluC+ LacC + SM + Gb, for MDCK cells, GluC+ SM for Jurkat cells, intensities taken from "C-labeled lipids), cholesterol and glycerophospholip- ids (PE + PI+ PS+ PC, intensities taken from "P-labeled lipids). Sphingolipid/ glycerophospholipid and cholesterol/glycerophospholipid ratios relative to the ratios for TCMS are shown. GPL, glycerophospholipids. with cont solu sess stre of pe for ber of carbon atoms and/or the number of double bonds in the fatty acid moicties, were detected. For cach species, its percent fraction of the total PC was calculated. In addition, the relative concentration of cach spccies was determined to allow compar- ison of the amounts present in different DRMS (see Table 1, which is published as supporting information on the PNAS web site, www.pnas.org). PC spccies were then grouped according to the degree of saturation of their fatty acids (Fig. 5). In MDCK cells,-60% of the total cell membrane PC was monounsaturated, <10% was fully saturated, and -30% pos- unsa dete Int of sa with noun from partit Jurka portic domai of gly results ferent Take MDCK cells TCM 70 Tween 20 as qua ent det by the 60 Bry 50 Lubral WX 50 Brj 98 prepara arbitrary units TCM Tween Brij 58 Lubrol Brij 98 Brij 96 Triton CHAPS TCM Tween Brij 58 Lubrol Brij 98 otal PC Brij 96 Triton CHAPS
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