Lysozyme is placed in a dialysis tube. The protein has a charge of +18 at pH 7.0. If the protein is dialyzed against 0.1L of 0.1M NaCl, predict the sign of the membrane potential using the Donnan Potential.
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. Lysozyme is placed in a dialysis tube. The protein has a charge of +18 at pH 7.0. If the protein is dialyzed against 0.1L of 0.1M NaCl, predict the sign of the membrane potential using the Donnan Potential.
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- List 4 different actiated carrier molecules, for each one, list both the oxided and reduced formsAnalysis of a protein is taking place. The enzymic acivity of this protein is stable up to temperatures of 40 degrees celsius. ph values are between 2.5 and 11.5. Now, Ion exchange chromatography is conducted via a software using DEAE-cellulose and method of elution is done by salt gradient. pH is set to 7. In terms of Molar, start of gradient is 0 and end of gradient is 1. The following graph is generated. Using the graph and analytical methods, determine pI of protein.Human serum transferrin is an iron transport protein. With the use of appropriate diagrams, describe the metal-bound active site and discuss the structural changes that occur on iron binding to the protein.
- Based on the Hill Plot below, this protein-ligand interaction displays log Y 1-Y 5 2.5 0 -2.5 -2.5 nH1 2.5 Binding that obeys the Law of Mass Action Negative cooperativity 5One of the important uses of the Nernst equation is in describing the flow of ions across plasma membranes. Ions move under the influence of two forces: the concentration gradient (given in electrical units by the Nernst equation) and the electrical gradient (given by the membrane voltage). This is summarized by Ohms law: Ix=Gx(VmEx) which describes the movement of ion x across the membrane. I is the current in amperes (A); G is the conductance, a measure of the permeability of x, in Siemens (S), which is I/V;Vm is the membrane voltage; and Ex is the equilibrium potential of ion x. Not only does this equation tell how large the current is, but it also tells what direction the current is flowing. By convention, a negative value of the current represents either a positive ion entering the cell or a negative ion leaving the cell. The opposite is true of a positive value of the current. a. Using the following information, calculate the magnitude of Na [ Na+ ]0=145mM,[ Na+ ]i=15mM,Gna+=1nS,Vm=70mV b. Is Na+ entering or leaving the cell? c. Is Na+ moving with or against the concentration gradient? Is it moving with or against the electrical gradient?The sodium/calcium exchanger (NCX) transports sodium into and calcium out of cardiac muscle cells. Describe why this transporter is classified as secondary active transport.
- It is typically sufficient to rupture cells when the solute concentration is reduced from 0.15M to 0.001M. Calculate what transmembrane pressure this would result in. Use that to access if the red blood cells would break. Yes or No? Compare to the transmembrane pressure when cells are in normal saline solution (0.91%NaCl) -> 0.156M(change unit to osM) Basically Calculate the transmembrane pressure when the solute concentration is reduced from 0.15M to 0.001M Determine if that transmembrane pressure would result in the breakage of red blood cells Calculate the transmembrane pressure when cells are in a normal saline solution and compareExplain the use of passing the inside-out vesicles contaminated with right-side-out vesicles through the affinity column made of lectin coupled to solid beads.A tetrapeptide, glutamate-glycine-alanine-lysine, is prepared at at concentration of 1 mM (0.001 M) and is measured in the standard setup (pathlength of 1 cm). What is the approximate absorbance of this peptide at 280 nm? Hint: if the peptide contained a single tryptophan, the answer would be about 10. 10 280 1 0
- the sodium channel exchanger NCX transports sodium into and calcium out of cardiac muscle cells. Describe why this itransporter is classified as secondary active transport?In the stomach, parietal cells are responsible for the formation of the gastric juice. During acid secretion, the pH in the stomach is estimated to be pH = 2, whereas parietal cells maintain an intracellular pH =7.35. The transmembrane potential of parietal cells is typically -70 mV. Body temperature 37°C. 1) Calculate the proton gradient concentration across the parietal membrane 2) Calculate the free energy change associated with the secretion of 1 mole of H* 3) Do you think that Ht transport can be driven by ATP hydrolysis at the ratio of one molecule of ATP per H* transported? You can use your textbook or other sources to check AG for ATP hydrolysis 4) If H* where free to move back to into the cell, calculate the membrane potential that would be required to prevent them to do soPlease ASAP. Thank you. Hyperpolarization activated cyclic nucleotide modulated channels (HCN). How is this channel activated? What is predominant ion that flows through this channel?
