Light-induced degradation of molecules, also called photobleaching, is a serious problem in applications that require very high intensities. A molecule of a fluorescent dye commonly used to label biopolymers can withstand about 106 excitations by photons before light-induced reactions destroy its π system and the molecule no longer fluoresces. For how long will a single dye molecule fluoresce while being excited by 1.0 mW of 488 nm radiation from a continuous-wave laser? You may assume that the dye has an absorption spectrum that peaks at 488 nm and that every photon delivered by the laser is absorbed by the molecule.
Light-induced degradation of molecules, also called photobleaching, is a serious problem in applications that require very high intensities. A molecule of a fluorescent dye commonly used to label biopolymers can withstand about 106 excitations by photons before light-induced reactions destroy its π system and the molecule no longer fluoresces. For how long will a single dye molecule fluoresce while being excited by 1.0 mW of 488 nm radiation from a continuous-wave laser? You may assume that the dye has an absorption spectrum that peaks at 488 nm and that every photon delivered by the laser is absorbed by the molecule.
Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter18: Raman Spectroscopy
Section: Chapter Questions
Problem 18.5QAP
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Light-induced degradation of molecules, also called photobleaching, is a serious problem in applications that require very high intensities. A molecule of a fluorescent dye commonly used to label biopolymers can withstand about 106 excitations by photons before light-induced reactions destroy its π system and the molecule no longer fluoresces. For how long will a single dye molecule fluoresce while being excited by 1.0 mW of 488 nm radiation from a continuous-wave laser? You may assume that the dye has an absorption spectrum that peaks at 488 nm and that every photon delivered by the laser is absorbed by the molecule.
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