Leave this Sordaria cross plate out of direct sunlight at 22-24 degrees C for 8-10 days. After this incubation the mature Sordaria cross plate cultures will look like the image on the right above. Use a toothpick or sterile loop to remove several ascocarps (see page 2 of the pre-lab file) from one of the hybrid zones. Make a wet mount of the ascocarps on a microscope slide and gently press the coverslip with your thumb or the eraser on the end of a pencil until the ascocarps are broken open. This will release clusters of asci. Place the slide under the microscope and scan the slide looking for clusters of released asci. Identify hybrid asci (with both dark and tan spores). Once you have found a cluster of hybrid asci score as many asci as possible for the arrangement of dark and light spores looking for asci where crossing over has taken place (see pre lab file) and below: No crossing over: Crossing over: For the asci shown in the figure on the next page: count the total number of asci, and count the number of asci showing crossing over. Calculate % recombination and the gene to centromere map distance: (# of asci with crossing over/total # asci)/2 X 100 00000000 00000 000 00000000 000000 200000000

You have 1 ml of kombucha. You remove 0.1 ml of this and mix it with 9.9 ml of fresh tea. You mix the second tube, then remove 1 ml of this mixture and add it to 9 ml of fresh tea. You then mix this, and remove 0.1 ml of this solution, and plate it. The next day, you return to find 362 colonies. What was the concentration of the cells in the original mix? Show your calculations.  My professor worked this problem out in class but she used a different method than that from the Khan academy videos on youtube and I am getting lost in the steps. Do you mind labeling?

Select the organism that matches each description. Some answers may be used more than once, and some not at all. Make sure that you scroll in the selection box, there are 13 possible matches. Was on Romaine lettuce sent from Yuma, AZ causing food-borne diarrhea Forms chains of Gram positive cells and is a common cause of tooth decay. A member of the normal vaginal flora that can overgrow at high pH Protozoan that is resistant to normal levels of chlorine used to disinfect the water Is salt tolerant and can inhabit the skin of healthy individuals, but can cause furunculosis Known as group B streptococci, is beta hemolytic and CAMP test positive Protozoan in stagnant water causing primary amoebic meningitis Psychrotropic, Gram positive rod transmitted in dairy causing systemic disease Escherichia coli O157:H7 Streptococcus pneumoniae Candida albicans Pseudomonas aeruginosa Cryptosporidium parvum Streptococcus mutans Streptococcus agalactiae Staphylococcus aureus Campylobacter jejuni A

Describe the results for each test indicated below. Use full sentences and write your answers in proper paragraph form. Gram stain Positive or Negative (How did you know?) Cell arrangement Cell morpholog Phenol Red Fermentation Did fermentation occur in each sugar? What products were produced? (How could you tell?) Streak for Isolation Successful or unsuccessful? (How could you tell?) Pigmented or non-pigmented? describe any other characteristics of Colony Morphology

__________ indicates ________________ in the cell culture.       Bovine Serum, trypsin       Yellow media, waste buildup       Orange media, contamination       Clear pink media, cell suspension

How would you modify this design to be a lateral flow strip that carries out DNA-based test? Use diagram below