Leave this Sordaria cross plate out of direct sunlight at 22-24 degrees C for 8-10 days. After this incubation the mature Sordaria cross plate cultures will look like the image on the right above. Use a toothpick or sterile loop to remove several ascocarps (see page 2 of the pre-lab file) from one of the hybrid zones. Make a wet mount of the ascocarps on a microscope slide and gently press the coverslip with your thumb or the eraser on the end of a pencil until the ascocarps are broken open. This will release clusters of asci. Place the slide under the microscope and scan the slide looking for clusters of released asci. Identify hybrid asci (with both dark and tan spores). Once you have found a cluster of hybrid asci score as many asci as possible for the arrangement of dark and light spores looking for asci where crossing over has taken place (see pre lab file) and below: No crossing over: Crossing over: For the asci shown in the figure on the next page: count the total number of asci, and count the number of asci showing crossing over. Calculate % recombination and the gene to centromere map distance: (# of asci with crossing over/total # asci)/2 X 100 00000000 00000 000 00000000 000000 200000000
Leave this Sordaria cross plate out of direct sunlight at 22-24 degrees C for 8-10 days. After this incubation the mature Sordaria cross plate cultures will look like the image on the right above. Use a toothpick or sterile loop to remove several ascocarps (see page 2 of the pre-lab file) from one of the hybrid zones. Make a wet mount of the ascocarps on a microscope slide and gently press the coverslip with your thumb or the eraser on the end of a pencil until the ascocarps are broken open. This will release clusters of asci. Place the slide under the microscope and scan the slide looking for clusters of released asci. Identify hybrid asci (with both dark and tan spores). Once you have found a cluster of hybrid asci score as many asci as possible for the arrangement of dark and light spores looking for asci where crossing over has taken place (see pre lab file) and below: No crossing over: Crossing over: For the asci shown in the figure on the next page: count the total number of asci, and count the number of asci showing crossing over. Calculate % recombination and the gene to centromere map distance: (# of asci with crossing over/total # asci)/2 X 100 00000000 00000 000 00000000 000000 200000000
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:多
Transcribed Image Text:ascocarps from along the petri dish
perimeter.
Leave this Sordaria cross plate out of direct sunlight at 22-24 degrees C for 8-10 days. After this
incubation the mature Sordaria cross plate cultures will look like the image on the right above. Use a
toothpick or sterile loop to remove several ascocarps (see page 2 of the pre-lab file) from one of the
hybrid zones. Make a wet mount of the ascocarps on a microscope slide and gently press the coverslip
with your thumb or the eraser on the end of a pencil until the ascocarps are broken open. This will
release clusters of asci. Place the slide under the microscope and scan the slide looking for clusters of
released asci. Identify hybrid asci (with both dark and tan spores). Once you have found a cluster of
hybrid asci score as many asci as possible for the arrangement of dark and light spores looking for asci
where crossing over has taken place (see pre lab file) and below:
No crossing over:
Crossing over:
For the asci shown in the figure on the next page: count the total number of asci, and count the number
of asci showing crossing over. Calculate % recombination and the gene to centromere map distance:
(# of asci with crossing over/total # asci)/2 X 100
00000000
00000 000
00000000O
00000000
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