Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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Pure Culture Techniques
When we try to study the bacterial flora of the body, soil, water, or just about any environment,
we realize that bacteria exist in natural environments as mixed populations. It is only in very
rare instances that they occur as a single species.
Pure cultures are required to study the cultural, morphological, and physiological
characteristics of an individual organism. Several methods for obtaining pure cultures are
available to the microbiologist today. Two commonly used methods are
• streak plate methods and
• pour plate methods.
Both types of procedures involve repeatedly diluting the cells in a sample to an end point
where a single cell divides giving rise to single pure colony. The colony is therefore assumed
to be the identical progeny of the original cell and can be picked and used for further study of
the bacterium.
Streaking
One drop of bacterial solution contains millions of cells. To identify a species, individual strains
have to be grown separately, starting from a single cell. The most commonly, used method to
isolate strain originating from a single cell is called the streaking method. There are several
different ways to streak a plate.
Figure 1: Streaking pattern on the agar plate. The colored lines indicate the path of the wire loop.
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Transcribed Image Text:Pure Culture Techniques When we try to study the bacterial flora of the body, soil, water, or just about any environment, we realize that bacteria exist in natural environments as mixed populations. It is only in very rare instances that they occur as a single species. Pure cultures are required to study the cultural, morphological, and physiological characteristics of an individual organism. Several methods for obtaining pure cultures are available to the microbiologist today. Two commonly used methods are • streak plate methods and • pour plate methods. Both types of procedures involve repeatedly diluting the cells in a sample to an end point where a single cell divides giving rise to single pure colony. The colony is therefore assumed to be the identical progeny of the original cell and can be picked and used for further study of the bacterium. Streaking One drop of bacterial solution contains millions of cells. To identify a species, individual strains have to be grown separately, starting from a single cell. The most commonly, used method to isolate strain originating from a single cell is called the streaking method. There are several different ways to streak a plate. Figure 1: Streaking pattern on the agar plate. The colored lines indicate the path of the wire loop.
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