Isoleucine has pką values of 2.32 (-COOH) and 9.76 (-NH3*). At which pH would isoleucine migrate towards the negative electrode during electrophoresis? O a. 9.76 O b. 2.32 O c. 6.24 O d. 7.65

Shown below is the amino acid tyrosine in its protonated state, with the pK of each ionizable proton indicated. pK = 10 HO H₂N pK = 9 COOH pK = 2 Which best represents the predominant state of tyrosine at pH 7? a HO b B C HO d HO Time left 0:20:16 H.N COOH HN COO HN COO HN COO

The pKa values for Lysine are 1.92/ 9.99/13.8.   At what pH will the amino acid not migrate in an electrical field? Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a 10.9 b 9.8 c 11.9 d 9.7 e 8.4 f 11.2 g 10.6 h 11.6 i 11.1   im thinking answer is c please clarify

You are super jazzed because your protein is purified and at a concentration of 600 μg in 1.75 ml of buffer. Determine the concentration in mg/ml. Show your calculation.

Could someone explain what this is saying? I would like a better understanding. FIGURE D CPP32 (Caspase-3) protease activity. Aliquots of cell lysates (50μL) were incubated with an equal volume (50 μL) of the reactionbuffer and 5 μL of 7-amino-4-trifluoromethyl coumarin (AFCDEVD) for 1 hour at 37˚C (Clontech). The shift in fluorescenceemission of AFC-DEVD, on its proteolysis to free AFC by theprotease, was detected in a fluorometer, using a 400 nm excitationfilter and 505 nm emission filter.

QUESTION 4 The table below shows the pka values for the various functional groups on cysteine. In which of the following forms will cysteine predominantly be found at pH 9.1? group pKa carboxyl (-COOH) 1.96 sulfhydryl (-SH) 8.18 ammonium (-NH3*) 10.13 А. В. CH,- SH CH,- SH H,N- CH- COOH H,N- CH- COo* | С. D. CH,-S CH,-S H,N - CH COO H,N- CH-COO O A OB OD

At what pH will the amino group of threonine be 50% deprotonated? The pKa values of threonine are 2.2, 9.8 and 13 O 13 O 56 O 22

The pKa for the side chain amino group in lysine is 10.4. If we were working at physiological pH 7.4, what would be the ratio of the protonated to unprotonated versions of lysine?   a. 1000:1   b. 3:1   c. 1:1000   d. 1:3

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what is the role of Cu+2 ion in biuret test for proteins?