Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Question
Is the inhibitor competitive or uncompetitive or non-competitive and why?
Notice that the Vmax value of 0.309407 will not be changed.
Your worksheet and graph should look similar to the figure below.
Michaelis Menten Plot.ls [Compatibility Mode]- Microsol
Home
Insert
Page Layout
Formulas
Data
Review
View
Add-Ins
Acrobat
% Cut
Calibri
11
AA
Wrap Text
General
Ce Copy
EE I Merge & Center
Conditional Forma
Formatting as Tabl
Paste
Format Painter
BIU
$- %
Clipboard
Font
Alignment
Number
C16
B
D
G
K
M
1 [Ethanol]
Vo
Calc. Vo delta^2
0.007
0.06
0.037997 0.000484
0.35
3.
0.015
0.11
0.071402
0.00149
4.
0.031
0.16
0.118415 0.001729
0.3
0.068
0.21
0.178303 0.001005
0.25
0.1
0.23
0.206272 0.000563
0.2
0.28
0.247526 0.001055
0.2
8
0.3
0.29
0.265206 0.000615
• Vo
0.4
0.28
0.275029 2.47E-05
0.15
Calc. Vo
10
0.006965
0.1
11
Km
0.05
12
Vmax
0.309407
0.05
13
14
0.1
0.2
0.3
0.4
0.5
16
17
18
Fig. 8. Michaelis-Menten Plot using a value of 0.05 mM for Km.
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Transcribed Image Text:Notice that the Vmax value of 0.309407 will not be changed. Your worksheet and graph should look similar to the figure below. Michaelis Menten Plot.ls [Compatibility Mode]- Microsol Home Insert Page Layout Formulas Data Review View Add-Ins Acrobat % Cut Calibri 11 AA Wrap Text General Ce Copy EE I Merge & Center Conditional Forma Formatting as Tabl Paste Format Painter BIU $- % Clipboard Font Alignment Number C16 B D G K M 1 [Ethanol] Vo Calc. Vo delta^2 0.007 0.06 0.037997 0.000484 0.35 3. 0.015 0.11 0.071402 0.00149 4. 0.031 0.16 0.118415 0.001729 0.3 0.068 0.21 0.178303 0.001005 0.25 0.1 0.23 0.206272 0.000563 0.2 0.28 0.247526 0.001055 0.2 8 0.3 0.29 0.265206 0.000615 • Vo 0.4 0.28 0.275029 2.47E-05 0.15 Calc. Vo 10 0.006965 0.1 11 Km 0.05 12 Vmax 0.309407 0.05 13 14 0.1 0.2 0.3 0.4 0.5 16 17 18 Fig. 8. Michaelis-Menten Plot using a value of 0.05 mM for Km.
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