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Inquiry In a cross between a wild-type female
fruit fly and a mutant white-eyed male, what color
eyes will the F1 and F2 offspring have?
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- Give typing answer with explanation and conclusion Consider a pair of homologous chromosomes heterozygous for three genes (e.g. ABC / abc) during prophase I of meiosis. Let the sister chromatids of one homolog be numbered 1 and 2; and the sister chromatids of the other homolog be numbered 3 and 4. A crossover that would result in genetic recombination (e.g., Abc or aBC) could involve which chromatids? a. 1 & 2 or 3 & 4 b. 1 & 3 or 2 & 4 c. 1 & 4 or 2 & 3 d. 1 & 3 or 1 & 4 or 2 & 3 or 2 & 4 e. any two of the four chromatidsLinkage Mapping Using a Trihybrid Testcross in Fruit Flies Remember the black, purple, and vestigial genes from Exercise 1? Now you're going to have to construct a map of the same region from trihybrid testcross data. The new map should be similar to the one from Exercise 1, but it won't be identical because we're starting with a new, independent data set. BLACK PURPLE VESTIGIAL TRIHYBRID TESTCROSS In the parental generation, you mate a pure-breeding wild-type female (bl+/bl*pu*/pu+vg+/vg+ with a pure-breeding black, purple, vestigial male (bl/bl;pu/pu vg/vg) to produce an F1 generation that is all wild-type (bl/bliput/pu;vg+/vg). Note that the F1 flies are all trihybrid. Next, you mate several F1 trihybrid females (bl/bl:put/pu;vg+/vg) with tester males, which are black, purple, vestigial (bl/bl;pu/pu;vg/vg). The offspring of this trihybrid testcross are: Phenotype Wild-type Black, purple, vestigial Vestigial Black, purple Purple, vestigial Black Purple Black, vestigial bl pu vg bl pu…Let's say that an experiment resulted in the production of several unattached kinetochores. What effect, if any, would this have on (i) the duration (length) of the mitotic phase, (ii) the timing of cyclin B’s appearance or disappearance, and (iii) the period of time for which MPF is active? Briefly explain your reasoning.
- Linkage Mapping Using Dihybrid Testcrosses in Fruit Flies In fruit flies, the normal honey-gray body-color (bl*) is dominant to black (bl), having normal red eyes (pu+) is dominant to purple (pu), and having normal wings (vg+) is dominant to vestigial (vg). The three genes are located close together on the same chromosome. Your task is to use data from three dihybrid testcrosses to (1) calculate recombination frequencies, (2) convert recombination frequencies into map distances, and (3) build a map of the chromosome interval covered by the three genes, indicating their order and distances between them. BLACK PURPLE DIHYBRID CROSS In the parental generation, you mate a pure-breeding wild-type female (bl+/blt;put/pu+) with a pure-breeding black, purple male (bl/bl;pu/pu) to produce an F1 generation that is all wild-type (bl+/bl;pu*/pu). Note that the F1 flies are all dihybrid. Next, you mate several F1 dihybrid females (bl+/bl;pu*/pu) with tester males, which are black, purple…Early C. elegans embryos display mosaic determination, whereas early mouse embryos exhibit regulativedetermination. Predict the results you would expect ifthe following treatments were performed on four-cellembryos of each of these two species (assuming thesemanipulations could actually be performed):a. A laser is used to destroy one of the four cells (thistechnique is called laser ablation).b. The four cells of the embryo are separated fromeach other and allowed to develop.c. The cells from two different four-celled embryosare joined together to make an eight-celledembryo.Let's say that an experiment had no disruption to kinetochores and you are able to inactivate the CDC 14 phosphatase, which acts to remove phosphate groups from substrates that were previously phosphorylated by active MPF. What effect, if any, would this have on (i) the duration (length) of the mitotic phase, (ii) the timing of cyclin B’s appearance or disappearance, and (iii) the period of time for which MPF is active? Briefly explain your reasoning
- VISUALIZE Sketch a series of diagrams showing each of the following, making sure to end each series with haploid cells: (a)How a pair of alleles for a single locus segregate in meiosis (b)How the alleles of two unlinked loci assort independently in meiosis (c)How the alleles of two linked loci undergo genetic recombinationFig name and choose the option10:10 E ← sisAndMeiosis.docx FREE EDIT PDF FREE CONVERT PDF TO WORD 2. Why are chromosomes important? 3. How are meiosis I and meiosis II different? 1. What is the state of DNA at the end of meiosis I? What about at the end of meiosis II? 4. Why do you use non-sister chromatids to demonstrate crossing over? 7. Identify two ways that meiosis contributes to genetic recombination. 10. P FREE PDF FILLER 5. What combination of alleles could result from a crossover between BD and bd chromosomes? 8. Why is it necessary to reduce the number of chromosomes in gametes? 6. How many nuclei are present at the end of meiosis II? How many chromosomes are in each? a. Sperm Cell b. Egg Cell AP_5 c. Daughter Cell from Mitosis ON 5G I 9. Blue whales have 44 chromosomes in every cell. Determine how many chromosomes you would expect to find in the following: d. Daughter Cell from Meiosis II COUS ra PAGE 10... FREE CONVERT JPG TO PDF X OeScience Labs, 2016
- In an electrophoretic gel across which is applied a powerful electrical alternating pulsed field, the DNA of the haploid fungus Neurospora crassa (n = 7) moves slowly but eventually forms seven bands, which represent DNA fractions that are of different sizes and hence have moved at different speeds. These bands are presumed to be the seven chromosomes. How would you show which band corresponds to which chromosome?Topic: Gene Locus Height in gryphons is determined by a single locus. You cross a two 9' tall gryphons and get 1/4 10'; 1/2 9' and 1/4 8' tall gryphons. If you across two 7' tall gryphons you get 1/4 8', 1/2 7'; 1/4 6' tall gryphons. pls kindly help to explanation: why In gryphons, alleles of the height lous are semidominant and form an allelic series. A.You are investigating two temperature-sensitive mutant cells. In one, you can inactivate shugoshin activity by raising the temperature slightly at the start of meiosis. In the other, you can similarly inactivate separase activity by raising the temperature. How would the two cells be affected by inactivating these proteins?