In 1913 Michaelis and Menten (MM) made six assumptions to describe the relation between Vo and [So] which were to be the foundation of steady-state kinetics. Briefly discuss each assumption

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A set of biomolecules listed in the table at right are in solution at pH 6.8, when they are passed through the multi-step separation process shown below. The sample passes through an ultrafilter with MWCO of 50,000 (assume this filter gives a perfect separation of molecules above and below the MWCO), and the retentate then flows through an ion exchange column filled with beads that have a positive charge under the operating conditions (pks = 7.3). Inlet Biomolecule Mixture pH 6.8 Ultrafiltration MWCO 50,000 Permeate Retentate lon Exchange Column Biomolecule Molecular weight pl A 68,000 4.2 B 92,600 9.6 C 144,000 A-3. Which biomolecules (A, B, C, etc.) exit the process in the permeate stream? K C-3. Which biomolecules are retained inside the ion exchange column? D E F G H B-3. Which biomolecules exit the process after passing through the ion exchange column? 5,800 68,000 Packed with DEAE cellulose beads, which have positively charged groups below a pH of 7.3 156,000 45,000 16,000 None…

What is the Michaelis-Menten equation and what is it used for?

The following questions are based on the reaction A+ B ↔ C+D shown in Figure 8.1.   1. Which of the following terms best describes the progress of the reaction with respect to free energy change? a) endergonic, ∆G> 0 b) exergonic, ∆G> 0 c) exergonic, ∆G< 0 d) endergonic, ∆G< 0   2. Which of the following in Figure 8.1 remains unchanged by having an enzyme included? a) b b) d c) a       d) c   3. The part labeled “C” on the above graph represents a) Energy of activation without enzyme b) Energy of activation with enzyme c) Amount of free energy released d) amount of energy required for the reaction progress