Immunfluorescence for Confocal Microscopy

getty Ed Res • You view this cell with the 40x objective lens and it is 7 optical units wide. Use the table below to determine the size of this cell. 1 ocular unit = Objective lens 25 um 4 x 10 μη 10 x 2.5 um 40 x 100 x 1 µm

PART C: CALCULATING THE DIAMETER OF THE FIELD OF VIEW (FOV)_ The field of view (FOV) is the circular area you can see when you look through the microscope. The diameter of the field of view is different depending on which objective lens you are using. For example, you are using the medium-power objective lens, then the area you can see is actually smaller than if you were using the low-power objective lens. Knowing The diameter of the field of view can help you estimate actual size of objects / cells seen through the microscope. When the revolving nose piece is turned to the low power objective lens, a dear plastic ruler can be placed on the microscope stage (see figure 1). Then, the coarse adjustment knob can be used to focus on the millimeter marks of the ruler making sure that one of the milimeter marks is at the left edge of the field of view (see figure 2). NOTE: Slage cip I-1000 Objects in the FOV are usually measured in micrometers (um). To convert, a FOV in mm, times it by 1000…

Compare and contrast the operation of optical microscopy and TEM in terms of Brightness  Note: Wherever possible, explain what factors affect or control each features