II. Identify what the mRNA, tRNA and amino acid for the DNA bases below. DNA bases below can be translated to become the amino acid present in a cow. DNA bases mRNA (Transcripion) TRNA Codons (Translation) Amino Acids 1. CÁC 2. AAA 3. АСА 4. ATG 5. АТА 6. TTA 7. GTA 8. TTC 9. ТСС 10. ACT

2. Which of the following is a true statement concerning condons? Condon specifies one amino acid is found in all eukaryotes, but not in prokaryotes will be read by RNA polymerase during transcription is composed of two nucleotides is comprosed of three amino acids

2. DNA → AGA ACA TAA ATG CTC TTA ACA CTC ATC AGA CCA GCA CTC CGA TGA క MRNA → TRNA → protein >

2. The following DNA sequence corresponds to the complete coding sequence of a gene that codes for a three-amino acid polypeptide: 5’-ATGTCCATG-3 What are the first two anticodon sequences of the first two t-RNA used by the ribosome? A. 3'-GUA-5' & 3'-CCU-5' B. 3'-TAC-5' & 3'-AGG-5' 3'-GAU-5' & 3'-CCU-5' C. D. 3'-UAC-5' & 3'-AGG-5' E. 3'-CUA-5' & 3'-GGU-5'

1. Draw your own version of the Central Dogma below, being sure to include the terms translation, transcription and synthesis. 2. Fill in the following chart regarding the Central Dogma: Transcription Translation Goal (outcome) Required molecules Location where it Occurs Post-event modifications 3. Identify the three main types of RNA and explain the function of each. 4. What is the genetic code and what is it used for?

Which statement is CORRECT? the primary transcript of RNA is larger than the MRNA the primary transcript of RNA is the same size as the mRNA O the primary transcript of RNA is smaller than the mRNA O both the primary transcript of RNA and MRNA contain introns

4. Which MRNA sequence complements the DNA sequence below? (LS1- 1) C G O Sequence A O Sequence B Sequence C O Sequence D A,

Section 2: Protein Synthesis Protein synthesis is the process whereby a gene of interest is transcribed into a strand of mRNA, which is subsequently translated into an amino acid sequence that will ultimately fold into a functional and highly specific protein.

1. Draw a line representing mRNA that has already been processes (introns removed, cap and tail added). Label the 5' cap on left end and polyA tail. 2. An inch from the left add the start codon (AUG). 3. An inch from the right add one of the three stop codons. 4. Label the 5' UTR and 3' UTR (untranslated regions). 5. In the middle of your mRNA, draw two ovals representing the large and small subunits of the ribosome bound to the mRNA. Make these large enough that you can add the details below. In between the two subunits indicate the ribosomal A, P and E sites in the correct place. Put an arrow on top of ribosome to show direction of translation 6. Draw at least 3 tRNA on your picture in the correct locations: an empty one (no amino acid) exiting the ribosome, a charged tRNA with a small circle on the 3' end to indicate an amino acid, and a tRNA with several small circle representing amino acids in a chain (polypeptide). 7. Indicate the amino end of polypeptide with an “N”. (Remember…

Synthesize a Protein 5. Below is a region of a gene. Transcribe the gene into a pre-mRNA strand. DNA C C MRNA