If the chromatogram was developed from reverse phase conditions, which of the following amino acids is expected to elute first? serine O phenylalanine tryptophan tyrosine

Introductory Chemistry: A Foundation
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Chapter21: Biochemistry
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Question 28
Use the following chromatogram of standard amino acids:
24
11
16
19
3
12
21
15
6. 8 9 19
23
17
13
14
18
20
22
10
12
14
16
18
20
22
24
26
Time (min)
[Au)
Transcribed Image Text:Question 28 Use the following chromatogram of standard amino acids: 24 11 16 19 3 12 21 15 6. 8 9 19 23 17 13 14 18 20 22 10 12 14 16 18 20 22 24 26 Time (min) [Au)
A. Amino Acids with Electrically Charged Side Chains
Negative
Pesitive
Aspartie Acid Glutamie Acid
(Asp D
Lysine
Histidine
Arginine
(Argl ®
(Glul O
(His H
HO
Ho
HO
-NH,
-NH,
NH,
NH,
-NH,
NH
NH
H,N-
ONH,
ONH,
C. Special Cases
B. Amino Acids with Polar Uncharged Side Chains
Proline
Asparagine
(Aan N
Cysteine
(Cya) ©
Glycine
(Gly O
Threonine
Glutamine
(Pra) P
Serine
(Ser 9
Но
Họ
Ho
Ho
Ho
Но
NH,
NH,
NH
NH,
NH,
NH,
-NH,
но-
SH
он
NH,
NH,
D. Amino Acids with Hydrophobic Side Chains
Leucine
(Leu O
Phenylalanine
(Phe e
Tryptophan
(Tp W
Tyrosine
Methionine
Valine
Isoleucine
Alanine
(Ala A
IMe M
Ho
Ho
Ho
Ho
HO
NH,
-NH,
Но
-NH,
HO
NH,
NH,
NH,
NH,
NH,
NH
он
If the chromatogram was developed from reverse phase conditions, which of the following amino acids is expected to elute first?
serine
phenylalanine
tryptophan
O tyrosine
Transcribed Image Text:A. Amino Acids with Electrically Charged Side Chains Negative Pesitive Aspartie Acid Glutamie Acid (Asp D Lysine Histidine Arginine (Argl ® (Glul O (His H HO Ho HO -NH, -NH, NH, NH, -NH, NH NH H,N- ONH, ONH, C. Special Cases B. Amino Acids with Polar Uncharged Side Chains Proline Asparagine (Aan N Cysteine (Cya) © Glycine (Gly O Threonine Glutamine (Pra) P Serine (Ser 9 Но Họ Ho Ho Ho Но NH, NH, NH NH, NH, NH, -NH, но- SH он NH, NH, D. Amino Acids with Hydrophobic Side Chains Leucine (Leu O Phenylalanine (Phe e Tryptophan (Tp W Tyrosine Methionine Valine Isoleucine Alanine (Ala A IMe M Ho Ho Ho Ho HO NH, -NH, Но -NH, HO NH, NH, NH, NH, NH, NH он If the chromatogram was developed from reverse phase conditions, which of the following amino acids is expected to elute first? serine phenylalanine tryptophan O tyrosine
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