Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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- I have an aqueous solution of tyrosinase but I require the solid form of tyrosinase. How would you process the aqueous solution to obtain the solid form?
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- Why is trypsin an unusual name for an enzyme? (What is the convention for enzyme names?) .arrow_forwardOne sample of the globular protein peroxidase is hydrolyzed by heating with 6 M HC1. A second sample is denatured by treatment with acetone. a) How does the peroxidase structure change during hydrolysis? Will this change the biological activity of peroxidase? b) How does the peroxidase structure change during denatur-ation? Will this change the biological activity of perox-idase? c) Are either of these processes likely to be reversible?arrow_forwardIdentify the hydrogen bond donor and acceptor groups in asparagine.arrow_forward
- Which of the following best describes the molecule below? . CHOH H H CH OH H OH OH CH OH OH H. OH B -D-galactopyranosyl-(104)-D-glucopyranose a -D-glucopyranosyl-(102)-D-fructofuranoside a -D-glucopyranosyl-(102)-D-glucofuranoside B -D-galactopyranosyl-(104)-D-fructopyranosearrow_forwardThe anomer of a-D-glucopyranose is CH:OH он ÓH CH2OH он он он он CH,OH он он он он CH,OH он OH он ÓH ÇH;OH он он он онarrow_forwardA toxin is glycopeptide, X that is composed of an octapeptide and a saccharide Y. Glycopeptide X is composed of a glycan Y and an octapeptide. The octapeptide was cleaved by trypsin, giving 2 tetrapeptides of exactly the same composition except for its N-terminals. When the octapeptide was cleaved by chymotrypsin, it gave a pentapeptide and a tripeptide. DNFB treatment gave DNP-ser. The composition of the octapeptide is lys, ser, trp.and leu. The leu:ser ratio is 4:1. 1. What is the sequence of this octapeptide? Saccharide Y is a tetrasaccharide. To determine its linkages, a person methylated glycopeptide X effectively methylating saccharide Y. Cleavage gave the peptide and the following products: 2,3,4-tri-O-methyl-a-D-glucopyranoside acid, 2,3-di-O-methyl-ß-D- glucopyranoside, 1,3,4-tri-O- methyl-ß-D-glucuronic acid treatments to the isolated tetrasaccharide gave the following results: and 1,4,6-tri-O-methyl-ß-D-N- acetylgalactosamine. Further 1. A. Treatment of saccharide Y with an…arrow_forward
- Which of the structures below is that of О-а-D-glucopyranosy-(1->1)- а-D-glucoругanoside CH2OH CH2OH O. H H. он H. он H OH ÓH CH2OH HOH2 H. CH2OH CH2OH O. H H. он он H OH он CH2OH H. о, он он H он онarrow_forwardthe following peptide is incubated as chymotrypsin: Gly-Val-Phe-Lys-Ala. Present the detailed chemical mechanism by which chymotrypsin hydrolyzes this peptide. Briefly explain each step. Include only the structure of the final products of the reaction.arrow_forwardWhy is a strong reducing agent, such as NaBH4, not suitable for use in the conjugation of an oligosaccharide to a protein?arrow_forward
- A) Can you please tell me which of these A.A have a negative side chain? Names of choices in picture provided. B) Can you please tell me which A.A have a hydrophobic side chain and why? Names and choices in picture provided. Thank You so much!!arrow_forwardDraw the structural formula for α-D-glucosyl-(1→6)-D-mannosamine,and circle the part of this structure that makes the compound a reducing sugar.arrow_forwardHow does dithiothreitol (DTT) differ from B-mercaptoethanol (BME) as a reducing agent?arrow_forward
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