Molecular weight Chemical 66.5 kDa Bovine serum albumin (BSA) 180 Glucose (C6H1206) 58.4 Sodium chloride (NaCl) Sodium citrate (Na3C6H507) 258 Sodium dodecyl sulfate (SDS, NaC 12 H2 5SO4) 288 Tris (C4H11NO3) 121.1 1. Calculate how much glucose you need to make 500 ml of a 5% glucose solution. 2. Calculate the molarity of the glucose solution you made in Q1. 3. How would you make 2 liters of a 0.5 M Tris buffer, pH 7.6? 4. How would you make 500 ml of 5 M NaCI? 5. How would you make 1 liter of 2 M sodium citrate? 6. How would you make 1 liter of stock 20X SSC (standard sodium citrate) buffer that is 3 M NaCl, 0.3 sodium citrate, using the solutions you made in Q4 and Q5 above? 7. Calculate how much SDS you need to make 800 ml of a 5% SDS solution. 8. Calculate how much SDS and SSC you need from the solutions made in Q6 and Q7 to make 1 liter of 0.1X SSC, 0.1% SDS. 9. How would you make 1 liter of 20 mM Tris, 10 mM NaCl from the solutions made in Q3 and Q4? 10. How much BSA would you need to add to the solution in Q9 to make it 1% BSA? 131% XPO W DOC MacBook Pro DII DD F6 F 7 F 8 F9 F10 F11 & 7 +
Molecular weight Chemical 66.5 kDa Bovine serum albumin (BSA) 180 Glucose (C6H1206) 58.4 Sodium chloride (NaCl) Sodium citrate (Na3C6H507) 258 Sodium dodecyl sulfate (SDS, NaC 12 H2 5SO4) 288 Tris (C4H11NO3) 121.1 1. Calculate how much glucose you need to make 500 ml of a 5% glucose solution. 2. Calculate the molarity of the glucose solution you made in Q1. 3. How would you make 2 liters of a 0.5 M Tris buffer, pH 7.6? 4. How would you make 500 ml of 5 M NaCI? 5. How would you make 1 liter of 2 M sodium citrate? 6. How would you make 1 liter of stock 20X SSC (standard sodium citrate) buffer that is 3 M NaCl, 0.3 sodium citrate, using the solutions you made in Q4 and Q5 above? 7. Calculate how much SDS you need to make 800 ml of a 5% SDS solution. 8. Calculate how much SDS and SSC you need from the solutions made in Q6 and Q7 to make 1 liter of 0.1X SSC, 0.1% SDS. 9. How would you make 1 liter of 20 mM Tris, 10 mM NaCl from the solutions made in Q3 and Q4? 10. How much BSA would you need to add to the solution in Q9 to make it 1% BSA? 131% XPO W DOC MacBook Pro DII DD F6 F 7 F 8 F9 F10 F11 & 7 +
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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How do you solve these? Please explain
Transcribed Image Text:Molecular weight
Chemical
66.5 kDa
Bovine serum albumin (BSA)
180
Glucose (C6H1206)
58.4
Sodium chloride (NaCl)
Sodium citrate (Na3C6H507)
258
Sodium dodecyl sulfate (SDS, NaC 12 H2 5SO4)
288
Tris (C4H11NO3)
121.1
1. Calculate how much glucose you need to make 500 ml of a 5% glucose solution.
2. Calculate the molarity of the glucose solution you made in Q1.
3. How would you make 2 liters of a 0.5 M Tris buffer, pH 7.6?
4. How would you make 500 ml of 5 M NaCI?
5. How would you make 1 liter of 2 M sodium citrate?
6. How would you make 1 liter of stock 20X SSC (standard sodium citrate) buffer that is 3 M NaCl, 0.3
sodium citrate, using the solutions you made in Q4 and Q5 above?
7. Calculate how much SDS you need to make 800 ml of a 5% SDS solution.
8. Calculate how much SDS and SSC you need from the solutions made in Q6 and Q7 to make 1 liter of
0.1X SSC, 0.1% SDS.
9. How would you make 1 liter of 20 mM Tris, 10 mM NaCl from the solutions made in Q3 and Q4?
10. How much BSA would you need to add to the solution in Q9 to make it 1% BSA?
131%
XPO
W
DOC
MacBook Pro
DII
DD
F6
F 7
F 8
F9
F10
F11
&
7
+
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