How would you design the experiment to determine the CFUs/ml of obligate anaerobic bacterium?

In Beadle  and Tatum experiments the immediate effect of the X-ray treatment was

During the Ames test, his− bacteria are exposed to a mixture of chemical compounds extracted from SurbWay Sandwich. If the his− bacteria revert to his+, then what can we say about the chemical?

In the Avery, McLeod, McCarty Experiment where supernatant from heat killed, virulent S Strain pneumonia solutions were added to non-virulent R Strain pneumonia cell cultures and allowed to grow in liquid media (i.e., broth). In tubes where Protease was added to the supernatant prior to cell culture, what was the observed effect when plating and growing the S. pneumonia cells to solid media?

1) The inactivation of Staphylococcus aureus was studied in pomegranate juice heated at 75°- 95°C. The slopes for the inactivation of this bacteria were calculated from the linear lines in semi-log graphical paper. The slope values were presented in Table 1. a) Determine the temperature change needed to inactivate this bacteria by 90%. b) Temperature change to inactivate Bacillus cereus in pomegranate juice by 90% at the same temperature range was 29°F. At 75°-95°C temperature range, which bacteria will be more affected by the temperature changes? Commend on your answer in one sentence Table 1- Slope values for the inactivation of Staphylococcus aureus in pomegranate juice heated at various temperature Temp. (°C) 75 80 85 95 -Slope x 10² (min-¹) 2.222 3.704 5.000 13.158