How would you design the experiment to determine the CFUs/ml of obligate anaerobic bacterium?

Q: 1 (a)Why is the spectrophotometer set at 0.000 absorbance for the uninoculated tube of broth? (B)…

A: A spectrophotometer is an instrument that measures the number of photons (light intensity) absorbed…

Q: The following set of data are measurements of zones of inhibition (in mm) from a Kirby-Bauer test.…

A: The Kirby-Bauer test, also known as the disc diffusion test, is a standard method used to determine…

Q: When testing the sensitivity of antibiotics using the Kirby-Bauer method, the Petri Dish with…

A: The Kirby-Bauer test, known as the disk-diffusion method, is the most widely used antibiotic…

Q: In Griffith's experiment, the S type bacteria are harmless, but can be transformed into the…

A: In Griffith's experiment, there was a mix of  heat-killed S-strain bacteria with live, harmless…

Q: Two different bacterial samples, A and B, were inoculated onto SIM media. What can you conclude…

A: SIM (Sulfide, Indole and Motility) test is done to differentiate the Gram negative bacilli based on…

Q: If you didn’t get countable colonies and are not able to calculate CFU, consider the possible…

A: In laboratories,  the bacterial cultured on the agar plates are counted by the help of CFU(colony…

Q: What information do you get from these controls to give you confidence in your results? How does…

A: Microorganism are small organism which we can observed through the microscope. It is usually used as…

Q: You take 10 ml of a stock solution, which is at a concentration of 1000 phage/ml, and dilute it to a…

A: A serial dilution is a laboratory technique that uses a series of consecutive dilution steps to…

Q: Give two ways to prevent the diffusion of oxygen into the medium in this test.

A: The main two ways to prevent the diffusion of oxygen are given below.

Q: What salt did Meselson and Stahl use for their ultracentrifuge gradients? O NaCI O NH4CI O CSCI O…

A: Given: DNA replicates semiconservatively. Matthew messelson and Franklin stahl performed an…

Q: What are challenges or difficulties we may encounter for identifying a bacillus cereus microbe ?

A: Bacillus cereus is a gram-positive, facultatively anaerobe, and the rod-shaped bacterium present in…

Q: explain why it is important to maintain sterility when inserting a foley cathether

A: Foley's catheter is an indwelling catheter, i.e. it can be left in place for longer durations than…

Q: The volume of E. coli added to each warm agar pour/virus mixture was originally100µL.  After…

A: E.coli when added to the agar pour containing virus was originally 100 ul and later changed to 300…

Q: What is the purpose of the negative and positive control in the Disc diffusion assay? Why is it…

A: The disc diffusion assay is used to determine the susceptibility of test bacteria to different…

Q: Define aseptic technique and briefly explain what we should do in the lab to provide aseptic…

A: The main aim of aseptic technique is to maintain a sterilized area in the lab and to control spread…

Q: If you increase the sample size (n) of a sample, will the following values increase, decrease,…

A: Answer :  1. When n increases, the standard error of the sample mean will decrease. This is due to…

Q: Complete the following questions for your gram negative rod unknown organism (a) What test did you…

A: The gram-negative rod group is a diverse and extensive category of bacteria characterized by their…

Q: -Two hundred patients without chest pain were screened with a new biochemical test that showed 190…

A: A screening test is important in the diagnosis of any disease at the initial stage. In some cases,…

Q: Why did the blank used in experimental procedures contain substrate, buffer and water?

A: A blank or blank determination is an analysis without a sample (Enzyme). Any enzyme assay requires a…

Q: Describe in detail the process of electrospray ionization ?

A: Ionisation is done by high voltage. Ions spread evenly on a surface. Ionised liquid gives rise to…

Q: what is the purpose of the FeCl3 in this aspirin test?

A: Now, setting up and observing the breakdown:1) Divided: We split apirin into two tubes, one with HCl…

Q: In the Avery, McLeod, McCarty Experiment where supernatant from heat killed, virulent S Strain…

A: In this talk, we will learn about a very important experiment called the Avery, McLeod, and McCarty…

Q: You perform a series of 4 different dilutions of 200 µL of sample into 800 µL of saline. What is the…

A: Dilution factor can be calculated using the formula:C1V1=C2V2

Q: ow do you figure out a number of live cells using flow cytometry results?

A: Flow Cytometry is a technique used to determine and analyze the characteristics of cells using…

Q: You got an opportunity to join a professor lab who is working in-vivo model and specifically looking…

A: Mitochondria are called the power house of the cells as it is the site where the maximum amount of…

Q: What is the concentration of a 50% solution after completing three 1:10 serial dilutions?

A: Serial dilution is the process that reduces the concentration of a solution in an organised manner.…

Q: Use the image below to answer this question and the three questions that follow:   a) what is the…

A: Gel electrophoresis is the technique of separation of molecules on the basis of their size and…

Q: A genetically modified bacterium was cultivated to produce 1,3-propanediol (1,3-PDO), and the…

A: Oxygen Demand is a measure of the amount of oxidizable substances in a water sample that can lower…

Q: You perform the Methyl-Red(MR)& Voges-Proskauer(VP)test and gather the followingresults:…

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: A) Identify the INDEPENDENT variable in the experimental setup shown in the diagram. B) Describe at…

A: Independent variable -  It is the variable that is controlled by the experimenter. It is the…

Q: syringes below, answer these six questions: QUESTIONS 1. Which syringe had the most floating disks?…

A: By the data given in the question we can see that this experiment is very much related to…

Q: What will be the results of the following experiment with explanations: A. Gram-positive bacteria…

A: The response of Gram-positive and Gram-negative microscopic organisms is examined in this experiment…

Q: ii) State what is happening at those wavelengths suggested in (i) above to encourage accumulation of…

A: Light and Pigments Engelmann's Experiment -- Engelmann strung one filament of algae on a slide under…

Q: You have several different media onto which you inoculated eight strains of yeast (A-H). The media…

A: Minimal medium (unsupplemented) A minimal medium is a growth medium used for growing microbes and…

Q: What is Bacterial Injury in Microbiology?

A: Bacteria produced in pure culture under ideal circumstances are examined in the lab for their…

Q: Explain how to conduct a Kirby-Bauer disk diffusion test. Include all of the steps involved

A: INTRODUCTION Kirby - Bauer Disc diffusion method Kirby - bauer disc diffusion method is widely known…

Q: What is the independent variable? What is the dependent variable? Suggest a control for this…

A: Lactobacillus bacteria are found in rich carbohydrates containing products like milk, cheese,…

Q: The Kirby-Bauer disk diffusion method is often used in clinical diagnostic labs.   Question 2…

A: Antibiotics are defined as the class of the drugs which are used to kill or retard the growth of…

Q: How is the information from a Kirby-Bauer disk diffusion test used for the recommendation of the…

A: Disclaimer: Kindly repost for other questions. As per the guidelines we are supposed to answer only…

Q: By convention, when the difference between the observed experimental outcome and the expected…

A: As it is given that by convention biologists use the 5 %value to determine if observed deviations…

Q: List the mechanism and give several names of the following: Class I, Class II, Class III, and Class…

A:

Q: Provide two advantages and two disadvantage of flow cytometry analysis. Briefly explain each.

A: Introduction  This technique is used to detect and measure cells physical and chemical properties.…

Q: List down two important precautions in conducting brine solution experiment.

A: Brine solution experiment : The brine solution experiment is generally used to study the process of…

Q: Problem 5(classwork): a.)explain why certain sedative medicines which alter breathing can produce…

A: Sedatives are medications that are involved in lowering brain activity. They are used to relax the…

Q: The following questions refer to the analysis of cells using flow  cytometry  A) When analyzing…

A: Flow cytometry is a common cell biology technique using laser-based technologies in a heterogeneous…

Clinical Pharmacology

Pharmacology is a discipline of medicine that studies how medications interact with the systems and processes of living organisms, focusing on drug action mechanisms as well as therapeutic and other purposes.