How would you design the experiment to determine the CFUs/ml of obligate anaerobic bacterium?

Q: 1 (a)Why is the spectrophotometer set at 0.000 absorbance for the uninoculated tube of broth? (B)…

A: A spectrophotometer is an instrument that measures the number of photons (light intensity) absorbed…

Q: When testing the sensitivity of antibiotics using the Kirby-Bauer method, the Petri Dish with…

A: The Kirby-Bauer test, known as the disk-diffusion method, is the most widely used antibiotic…

Q: In Griffith's experiment, the S type bacteria are harmless, but can be transformed into the…

A: In Griffith's experiment, there was a mix of  heat-killed S-strain bacteria with live, harmless…

Q: Two different bacterial samples, A and B, were inoculated onto SIM media. What can you conclude…

A: SIM (Sulfide, Indole and Motility) test is done to differentiate the Gram negative bacilli based on…

Q: If the concentration of E. coli in a broth is between 104 and 106 cells per mL, the best way…

A: E.coli are the type of bacteria that is known to cause many illness in humans. E.coli is cultured in…

Q: You've been assigned with isolating a certain species of bacteria from a mixed culture in order to…

A: Introduction  Microbial isolation is a technique for growing microorganisms outside of their natural…

Q: If you didn’t get countable colonies and are not able to calculate CFU, consider the possible…

A: In laboratories,  the bacterial cultured on the agar plates are counted by the help of CFU(colony…

Q: What information do you get from these controls to give you confidence in your results? How does…

A: Microorganism are small organism which we can observed through the microscope. It is usually used as…

Q: In zone electrophoresis, why is it possible to isolate the components of a sample and recover them…

A: Introduction :- Electrophoresis is a laboratory technique used to separate and analyze DNA, RNA,…

Q: You take 10 ml of a stock solution, which is at a concentration of 1000 phage/ml, and dilute it to a…

A: A serial dilution is a laboratory technique that uses a series of consecutive dilution steps to…

Q: Give two ways to prevent the diffusion of oxygen into the medium in this test.

A: The main two ways to prevent the diffusion of oxygen are given below.

Q: What salt did Meselson and Stahl use for their ultracentrifuge gradients? O NaCI O NH4CI O CSCI O…

A: Given: DNA replicates semiconservatively. Matthew messelson and Franklin stahl performed an…

Q: What are challenges or difficulties we may encounter for identifying a bacillus cereus microbe ?

A: Bacillus cereus is a gram-positive, facultatively anaerobe, and the rod-shaped bacterium present in…

Q: The volume of E. coli added to each warm agar pour/virus mixture was originally100µL.  After…

A: E.coli when added to the agar pour containing virus was originally 100 ul and later changed to 300…

Q: What is the purpose of the negative and positive control in the Disc diffusion assay? Why is it…

A: The disc diffusion assay is used to determine the susceptibility of test bacteria to different…

Q: Define aseptic technique and briefly explain what we should do in the lab to provide aseptic…

A: The main aim of aseptic technique is to maintain a sterilized area in the lab and to control spread…

Q: Complete the following questions for your gram negative rod unknown organism (a) What test did you…

A: The gram-negative rod group is a diverse and extensive category of bacteria characterized by their…

Q: What is the difference between normal and reverse phase chromatography?

A: The main Difference between Normal and reverse phase chromatography is 1) In normal phase…

Q: Why did the blank used in experimental procedures contain substrate, buffer and water?

A: A blank or blank determination is an analysis without a sample (Enzyme). Any enzyme assay requires a…

Q: Describe in detail the process of electrospray ionization ?

A: Ionisation is done by high voltage. Ions spread evenly on a surface. Ionised liquid gives rise to…

Q: In step 3 of today's protocol, it mentions that the incubation step with the P2 buffer should not be…

A: P2 buffer is a lysis buffer solution containing sodium dodecyl sulfate to puncture holes in cellular…

Q: You perform a series of 4 different dilutions of 200 µL of sample into 800 µL of saline. What is the…

A: Dilution factor can be calculated using the formula:C1V1=C2V2

Q: A scientist transfers 0.1 mL phage stock to a tube containing 9.9 mL tryptic soy broth. She then…

A: The total fold of dilution represents how much the original concentration has been reduced due to…

Q: In the assay of citric acid, what do you think about the nature of solution sample ? a) Before…

A: In the citric acid assay, the concentration of the citric acid is used to determine with the help of…

Q: ow do you figure out a number of live cells using flow cytometry results?

A: Flow Cytometry is a technique used to determine and analyze the characteristics of cells using…

Q: When viewed under a microscope, bacteria appear as red rods after they have been stained. Explain a…

A: Introduction Bacteria Are Minute, Single-celled Organisms That Can Be Found In Large Numbers In All…

Q: A genetically modified bacterium was cultivated to produce 1,3-propanediol (1,3-PDO), and the…

A: Oxygen Demand is a measure of the amount of oxidizable substances in a water sample that can lower…

Q: You perform the Methyl-Red(MR)& Voges-Proskauer(VP)test and gather the followingresults:…

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: syringes below, answer these six questions: QUESTIONS 1. Which syringe had the most floating disks?…

A: By the data given in the question we can see that this experiment is very much related to…

Q: What will be the results of the following experiment with explanations: A. Gram-positive bacteria…

A: The response of Gram-positive and Gram-negative microscopic organisms is examined in this experiment…

Q: ii) State what is happening at those wavelengths suggested in (i) above to encourage accumulation of…

A: Light and Pigments Engelmann's Experiment -- Engelmann strung one filament of algae on a slide under…

Q: You have several different media onto which you inoculated eight strains of yeast (A-H). The media…

A: Minimal medium (unsupplemented) A minimal medium is a growth medium used for growing microbes and…

Q: What is Bacterial Injury in Microbiology?

A: Bacteria produced in pure culture under ideal circumstances are examined in the lab for their…

Q: What is the independent variable? What is the dependent variable? Suggest a control for this…

A: Lactobacillus bacteria are found in rich carbohydrates containing products like milk, cheese,…

Q: The Kirby-Bauer disk diffusion method is often used in clinical diagnostic labs.   Question 2…

A: Antibiotics are defined as the class of the drugs which are used to kill or retard the growth of…

Q: what is the clinical significance of determining whether or not a bacterium is acid-fast?   list the…

A: Acid-fast bacteria, also termed as acid-fast bacilli, are microbes resistant to decolorization by an…

Q: How is the information from a Kirby-Bauer disk diffusion test used for the recommendation of the…

A: Disclaimer: Kindly repost for other questions. As per the guidelines we are supposed to answer only…

Q: By convention, when the difference between the observed experimental outcome and the expected…

A: As it is given that by convention biologists use the 5 %value to determine if observed deviations…

Q: List the mechanism and give several names of the following: Class I, Class II, Class III, and Class…

A:

Q: elevated NaCl level. A yellow halo surrounds the growth, indicating the bacterium fermented sugar in…

A: Answer D. selective and differential medium

Q: Provide two advantages and two disadvantage of flow cytometry analysis. Briefly explain each.

A: Introduction  This technique is used to detect and measure cells physical and chemical properties.…

Q: List down two important precautions in conducting brine solution experiment.

A: Brine solution experiment : The brine solution experiment is generally used to study the process of…

Q: Problem 5(classwork): a.)explain why certain sedative medicines which alter breathing can produce…

A: Sedatives are medications that are involved in lowering brain activity. They are used to relax the…

Q: The following questions refer to the analysis of cells using flow  cytometry  A) When analyzing…

A: Flow cytometry is a common cell biology technique using laser-based technologies in a heterogeneous…

Q: A flow cytometer is constructed of many components. For good quantitative results how and how…

A: Flow cytometry is a powerful technique used in various fields of science and medicine to analyze and…

Clinical Pharmacology

Pharmacology is a discipline of medicine that studies how medications interact with the systems and processes of living organisms, focusing on drug action mechanisms as well as therapeutic and other purposes.