How would each manipulation of the leader region of the trp mRNA affect transcription of the E. coli trp operon? 1. Increasing the distance (number of bases) between the leader peptide gene and sequence 2 would ___________________ transcriptional attenuation of the trp operon. (increase/decrease/not change) 2. Increasing the distance between sequences 2 and 3 would ____________________ transcriptional attenuation of the trp operon. (increase/decrease/not change) 3. Removing sequence 4 would ________________ transcriptional attenuation of the trp operon. (cause/not change/prevent) 4. Changing the two Trp codons in the leader peptide gene to His codons would cause transcriptional attenuation of the trp operon to ____________________. (lose its sensitivity to histidine/increase its sensitivity to tryptophan/lose its sensitivity to tryptophan) 5. Eliminating the ribosome‑binding site for the gene that encodes the leader peptide would cause transcriptional attenuation of the trp operon to _________________________. (always occur/rarely occur/not change) 6. Changing several nucleotides in sequence 3 so that it can base‑pair with sequence 4 but not with sequence 2 would cause ___________________ attenuation of the trp operon. (no change in/loss of/constant)
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
How would each manipulation of the leader region of the trp mRNA affect transcription of the E. coli trp operon?
1. Increasing the distance (number of bases) between the leader peptide gene and sequence 2 would ___________________
transcriptional attenuation of the trp operon. (increase/decrease/not change)
2. Increasing the distance between sequences 2 and 3 would ____________________ transcriptional attenuation of the trp operon. (increase/decrease/not change)
3. Removing sequence 4 would ________________ transcriptional attenuation of the trp operon. (cause/not change/prevent)
4. Changing the two Trp codons in the leader peptide gene to His codons would cause transcriptional attenuation of the trp operon to ____________________. (lose its sensitivity to histidine/increase its sensitivity to tryptophan/lose its sensitivity to tryptophan)
5. Eliminating the ribosome‑binding site for the gene that encodes the leader peptide would cause transcriptional attenuation of the trp operon to _________________________. (always occur/rarely occur/not change)
6. Changing several
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