how to read this table? like for example, just by looking at the table, it was said that leptin has 16 kda with 15% gel. How did they able to determine that leptin needs 15% gel? The separation of molecules within a gel is determined by the relative size of the poresformed within the gel. Thus, the gel percentage depends on the size of your protein ofinterest.Protein size (kDa)Gel percentage (%)4-402012-451510-7012.515-1001025-2008

Introduction: Separation is brought about through the molecular sieving technique, based on the…

Answered: how to read this table? like for…

how to read this table? like for example, just by looking at the table, it was said that leptin has 16 kda with 15% gel. How did they able to determine that leptin needs 15% gel?

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

how to read this table? like for example, just by looking at the table, it was said that leptin has 16 kda with 15% gel. How did they able to determine that leptin needs 15% gel?

The separation of molecules within a gel is determined by the relative size of the pores
formed within the gel. Thus, the gel percentage depends on the size of your protein of
interest.
Protein size (kDa)
Gel percentage (%)
4-40
20
12-45
15
10-70
12.5
15-100
10
25-200
8

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