How and why we do molecular cloning of enzymes?

Q. Which of the following is a key difference between eukaryotic and prokaryotic genomes?   A. Physical separation of transcription and translation occurs in eukaryotes, but no separation occurs in prokaryotes. B. Genes are arranged in order in eukaryotes, but the order is random in prokaryotes. C. Near simultaneous transcription and translation occurs in eukaryotes, but they are strictly sequential in prokaryotes. D. Gene expression is highly regulated in eukaryotes, but there is a lack of control of gene expression in prokaryotes.

b. Explain how Nirenberg and Leder/Matthaei were able to create many of the codon/amino acids found in the Genetic Code (i.e. what was their experimental set- up, how did they know if the codons matched, what were the knowns, and finally what codons did they struggle with and why).

C. Two mutations are known in the population, one shaded in yellow and one in blue, shown on the TEMPLATE DNA sequence below. Ursula has a mutation in the yellow- shaded underlined nucleotide: instead of an A in this position, she has a T. 5' AATCATAACTCATTG 3' What type of mutation does Ursula have? Second position First position (5 000 0 UUU UUC phe UUA UUG COU leu CỰU CUC CUA COO CUG ADO AUU AUC ile nov AUA AUG met GUU GUC GUA GUG val UCU UCC UCA ser UCA UCG COU CCU cce CCC CCA pro CCA CCG ACU www ACC ACA ACG GCU GCC GCA GCG thr ala UAU tyr UAC UAA Stop UAG Stop CAU CAC his CAC CAA gin CAG AAU AAC asn AAC AAA lys AAG GAU GAC asp glu GAA GAG UGU UGC cys UGA Stop UGG trp CGU Missense Silent Nonsense Frameshift CGC CGA arg CGA CGG AGU AGC AGA AGG GGU GGC GGA GGG Initiation Termination ser arg gly DUAG DUMU DUAU DUAG Third position (3-end)