H₂N. OH + H₂N. OH enzyme

Metabolic pathways frequently contain reactions with positive standard free-energy values, yet the reactions still take place. How is this possible?

Differentiate between homotropic and heterotropic and explain how they modify the equilibrium between the T and R forms of an allosteric enzyme.

Which of the following is not an assumption made when evaluating Michaelis Menton kinetics? the reaction is happening at body temperature the substrate is in great excess of the catalyst measurement of initial velocity of the reaction reaction conditions are occurring under steady state

Reactions taking place in dilute solution obey the rules ofsimple reaction kinetics because their rates depend on diffusion. How does this approach relate to investigations of enzymes in living cells?

Explain the details in the individual steps in the Michaelis-Menten mechanism of converting substrates into products

Explain the krebs cycle. You must know the reactants, products and location for each step.

Compile a list of all the cofactors involved in adding or removing one-carbon groups in carbohydrate, lipid, and amino acid metabolism. Provide an example of a reaction that uses each cofactor.

Y Explain and show why phosphoenolpyruvateis a high-energy compound.

Give an example of one enzyme that participates in both glycolysis and gluconeogenesis.  Evaluate the structures of chemical reactants and products and the enthalpic and entropic contributions to Gibbs free energy change for the reaction.  Explain how the enzyme is able to catalyze the reaction (either direction.)