**Baker Part 2: Investigation into Illness Outbreak**The next day, the local newspaper reported an outbreak of "projectile vomiting" within the neighborhood surrounding the bakery.Public health microbiologists, through food surveys, discovered all those ill had bought and/or eaten cream pies or cream puffs made by the baker.The public health microbiologists cultured the cream from the cream puffs and pies, with results listed below:- **Blood Agar Plates:** Incubated aerobically. Notable clearing around colonies indicates hemolytic activity.- **Gram Stain:** Revealed round purple cells, indicating Gram-positive bacteria.- **Growth on Mannitol Salt Agar (MSA):** Exhibited growth, suggesting the presence of bacteria capable of tolerating high salt concentrations, often indicative of Staphylococcus species. Each step provides essential insights into identifying the bacterial species responsible for the outbreak. **Growth on MSA (Mannitol Salt Agar) below:**The image shows a circular petri dish with yellow growth, indicating bacterial colonies. The yellow color suggests fermentation of mannitol by certain bacteria.**Catalase Results:**The image depicts a catalase test result showing bubbles, indicating a positive catalase reaction. This typically signifies the presence of catalase-positive bacteria such as Staphylococcus species.**Question:**What do you think caused the outbreak of projectile vomiting?- ○ Enterotoxin made by *Listeria monocytogenes*- ○ Enterotoxin made by *Bacillus cereus*- ○ Enterotoxin made by *Staphylococcus aureus*- ○ Enterotoxin made by *Clostridium perfringens*- ○ Enterotoxin made by *Staphylococcus epidermidis*

Enterotoxins are kind of exotoxins that are produced by some bacterias. These enterotoxin are cytotoxic that kills the cells by altering the permeability of the cell membrane.The enterotoxins acts on intestinal wall. Many bacterias produces enterotoxins such as staphylococcus aureus and bacillus cereus. Staphylococcus aureus is a gram positive bacterium that produces enterotoxin B, which is associated with food-borne illness. It causes projectlier vomiting but no diarrhea. It is a severe type of vomiting in which the stomach contents are expelled several feets away. It usually comes in sudden and violent bursts. The cause of outbreak of projectlier vomiting is enterotoxin produced by staphylococcus auerus.