Green Rf = 0.89 Red Rf = 0.81 Blue Rf = 0.72 Yellow Rf = 0.77 Which colored pigment has the greatest affinity for the stationary phase? O yellow O red O green O blue

The amino acid sequence (primary structure) and 3D shape (tertiary structure) of the rhodopsin has been determined and the various protein domains have also been identified with precision (see figures below). The red arrow indicates the amino acid lysin (K) that binds to the oxygen of the retinol (indicated by the blue arrow). The purple arrow indicates the retinal bound to the rhodopsin. In healthy individuals, retinal is abundant and can binds to the rhodopsin, making it functional (light-sensitive). H3C CH3 CH3 CH3 cytoplasmic side Retinal C-I extracellular side 0000 MOO OOO 400 DERY 000 PONE ALMO VOO LGF LLOD VUEL CH3 HOO AD SLV GO04 FAV OCK 000 LOO GOF YO DOAA TOTO ⓇAD MOWE OV00 OPL ALA LEGE DOOD VOOO COP DES 000 600 GCO OV FOOD Rhodopsin (primary structure) C-II E-II COOH-terminal C-III RIVE OOM DOWO LWCO 40 DOVO FOX ON DO 000 D04 boooooo 000 000 100,00 ****** E-III NH₂-terminal Rhodopsin + retinal (tertiary structure) 6. This lysine (K) is in a transmembrane domain of rhodopsin…

TWO PART QUESTION, PLEASE ANSWER BOTH, THANKS! The data seen are 4 various absorption wavelengths and it’s relative intensities. A. Using the data of relative intensity for 4 various absorption wavelengths, create a graph of the expected relative intensity for their respective emission wavelengths. Make sure to include all title and axis B. In only 2 sentences, explain your reasoning in your created graph above

○ Transition state analog Uncompetitive Mechanism-based Affinity-based O Non-specific