Glucose is the major energy-yielding nutrient for most cells. Assuming a cellular concentration of 1 M (that is, 1 millimole/L), calculate how many molecules of glucose would be present in our hypothetical (and spherical) eukaryotic cell. (Avogadro’s number, the number of molecules in 1 mol of a nonionized substance, is 6.02 × 10 23 .)

6-73 In each case, tell which side (if either) rises and why. The solvent is water. A A Osmotic membrane (a) 1% glucose (b) 0.1 M glucose (c) 1 M NaCl (d) 1 M NaCl (e) 3% NaCl (f) 1 M NaBr (B) B 5% glucose 0.5 M glucose 1 M glucose 1 M K₂SO4 3% KC1 1 M KC1

The α-amylase (α-AM) enzyme secreted by Aspergillus has an isoelectric point of approximately 4.2. If the purification method in problem 1 operates at pH 6.5, would the protein carry a positive charge, negative charge, or net zero charge?

A set of biomolecules listed in the table at right are in solution at pH 6.8, when they are passed through the multi-step separation process shown below. The sample passes through an ultrafilter with MWCO of 50,000 (assume this filter gives a perfect separation of molecules above and below the MWCO), and the retentate then flows through an ion exchange column filled with beads that have a positive charge under the operating conditions (pks = 7.3). Inlet Biomolecule Mixture pH 6.8 Ultrafiltration MWCO 50,000 Permeate Retentate lon Exchange Column Biomolecule Molecular weight pl A 68,000 4.2 B 92,600 9.6 C 144,000 A-3. Which biomolecules (A, B, C, etc.) exit the process in the permeate stream? K C-3. Which biomolecules are retained inside the ion exchange column? D E F G H B-3. Which biomolecules exit the process after passing through the ion exchange column? 5,800 68,000 Packed with DEAE cellulose beads, which have positively charged groups below a pH of 7.3 156,000 45,000 16,000 None…

Suppose the major solutes in intact lysosomes are KCl (~0.1 M) and NaCl (~0.03 M). When isolating lysosomes, what concentration of sucrose is required in the extracting solution at room temperature (25oC) to prevent swelling and lysis? Calculate how much sucrose (342.3 g/mol) would you need to make 500 ml of this extracting solution.

Explain what the meaning of these distances is (e.g., from where to where?)Crystal structure of human monocyte chemotactic protein-2

A spheroidal bacterium with a diameter of 1.0 μm (micrometer, 1 μm = 10-6) contains 25,000 molecules of the protein hexokinase. What is the molar concentration of the protein inside the cell?