Given the following mRNA sequence, write the peptide sequence that will result from protein translation. Please indicate the correct directionality.
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- 1 10 20 30 40 50 60 70 -I---- 5' АTCGGTCТCGGCTACTACАТАAАСGCGCGCATATATCGAТАТСТАGСТАGСТАТCGGTCTAGGCTACTАC 3' TAGCCAGAGCCGATGATGTATTTGCGCGCGTATATAGCTATAGATCGATCGATAGCCAGATCCGATGATG I--------I--- --I--- --I------ --I--- -I---------I Promoter 80 90 100 110 120 130 140 -I---- 5' CAGGTATсGGTCTGATCTAССТAGCTTCTтсттстстстстсссссGCGGGGGCTGTACTATСATGCGTCG 3' GTCCATAGCCAGACTAGATCGATCGAAGAGAAGAGAGAGAGGGGGCGCCCCCGACATGATAGTACGCAGC -I- -I------- -I--- -I---------I RBS 150 160 170 180 190 200 210 -----I--- ---I-- ------I- ---I---------I---- -I---------I 5' тстCGGCTАСТАCGTAAACGCGCGCATATAтCGATATCTAGCТAGСТАТСGGTстCGGCTACTAсGTAAA 3' AGAGCCGATGATGCATTTGCGCGCGTATATAGCTATAGATCGATCGATAGCCAGAGCCGATGATGCATTT 220 230 240 250 --I----- ---I-- ------I--- -I 5' CCCTATTAGCATGGGTCATATTTGTGTCTGCTTGTTGGGT 3' GGGATAATCGTACCCAGTAGAAACACAGACGAAGAACCCA a. What are the nucleotides of the MRNA from gene Z? b. What are the amino acids encoded by gene Z? ( Vate Vhidden message of the cide: 5' - UGAUGAUGAUGAUGCAUGCUAACGAUUCCGCAAUGUCGAUAUCAAUACGUUGACC-3'HindII --- 5' GTC - GAC 3', HaeIII --- 5' CC - GG 3', EcoRI --- 5' G - AATTC 3' and BamI --- 5' CCTAG - G 3' 5' AGAATTCTTACGCCGGACGTACCTAGGTTTAGTCGACTC CGCCGCCCCTAGGGTCATCA 3' 3' TCTTAAGAATGCGGCCTGCATGGATCCAAATCAGCTGAGGCGGCGGGGATCCCAGTAGT 5' Number of pieces of DNA , and blunt end fragment (s), and sticky end fragment(s)
- cggcaggagc cccccgacct cccaggcgga ccgccctccc tccccgcgcg cgggttccgg gcccggcgag agggcgcgag cacagccgag gccatggagg tgacggcgga ccagccgcgctgggtgagcc accaccaccc cgccgtgctc aacgggcagc acccggacac gggcctcagcc actcctacat ggacgcggcg cagtacccgc tgccggagga ggtggatgtg ctttttaaca tcgacggtca aggcaaccac gtcccgccct actacggaaa ctcggtcagg Provide the primers for the nucleotide sequence above (GATA3)geneTGTCTAGTTGGCAATTGTCCTAGCCATACTGTUNSCRAMBLE THE WORDS pgyocntei ernviaca lpomcohailgro niaovrait csepsei tcrhrsaccaeisti sndonsictouui atrit lbhreaavoi tiarvnoia
- AGTGCAGCTC CAGCCGACCC CCGGGACGTA GACAAGGGCA GGCGCGCGGT 50 GAAGACTGCG GCCGGCCGCG TAGCCGCGCT GTGGTCCCGC TGGCCTTCTC 100 TTGGGCCGCG CACCCTCCGG ACCTGCGTCG GGATCCTCCC GGAGTCTCCC 150 GCTCTCTCCT CCCGGCTTGC GAACATGCGG CCCCTTAAGC CCGGCGCCCC 200 TTTGCCCGCA CTCTTCCTGC TGGCGCTGGC TTTGTCCCCG CACGGAGCCC 250 ACGGGAGGCC CCGGGGGCGC AGGGGAGCGC GCGTCACGGA TAAGGAGCCC 300 AAGCCGTTGC TTTTCCTCCC CGCGGCCGGG GCCGGCCGGA CTCCCAGCGG 350 CTCCCGGAGC GCAGAAATAT TCCCAAGAGA CTCTAACTTA AAAGACAAAT 400 TCATAAAGCA TTTCACAGGG CCGGTCACAT TTTCACCAGA ATGCAGCAAA 450 CATTTCCACC GACTCTATTA CAATACCAGG GAGTGCTCAA CGCCAGCTTA 500 TTACAAAAGA TGTGCTAGAT TGTTAACAAG ATTAGCAGTG AGTCCACTGT 550 GCTCCCAGAC CTAGCAAAAC TACCCTACAT TTCCTAAGAA TGTACATCTA 600 ATTTGAAGAA AAAGTGCCTC AAATCATGCA AAATGTAAAA AAAGATGAAA 650 TTTATATTTT TATGGATATT AAGATGAGTA AAATAAGAGA CTTCCCAGAA 700 ATAACTGGTT AGCTGTTTCC TGTCATAGAA TGGAGTCTTT CTTGCTTTAT 750 CTTTTTGTGT ATACAGTAAT TTATAATTTT GTAAAACAGA GTTTGAATCG 800 CATATTGAAA ATTAGATATT AAAAATTGTG…C UUU UUC Phe UUA UCU) UCC UCA UAU UAC Tyr UGU UGC Cys Ser UAA Stop UGA Stop A UAG Stop UGG Trp UUG FLeu UCG CUU CUC CCU] СС CAU1 CAC САC His САА Gln CGU CGC Arg Leu Pro CUA CUG ССА CGA CUG J CCGJ Which of the following tRNA anticodons could theoretically base pair effectively with the codons 5'-AAU-3' and 5'-AAC-3' through third-base CAG, CGG AGU AAUASN AGC AUU AUC File A AUA ACU АСС АСА AAC FAsn AAA Ser C Thr AGA Arg A AUG Met ACG AAG Lys AGG GCU] GCC GCA GCG GGU GGC Gly GGA GAU1 GUU GUC GUA GUG Asp GACJ GAA C Val Ala A GAG Glu GGG wobble? 5'-GGG-3' OI. 5'-AUG-3' II. 5'-GUU-3' I. 5'-CAT-3' IV. 5'-AAA-3 V. Third letter UCAG UUAG A, First letter5'-AATGCCTCAGCCGATCTGCCTCGAGTCAATCGA TGCTGGTAACTTGGGGTATAAAGCTTACCCATGGTATCGTAG TTAGATTGATTGTTAGGTTCTTAGGTTTAGGTTCTGGTATT GGTTTAGGGTCTTTGATGCTATTAATTCTTTGGTTTTGATTT GGTCTTTATATGGTTTATGTTTTAAGCCGGGTTTTGTCTGG - GATGGTTCGTCTGATGTGCGCGTAGCGTGCGGCG - - - 3' What are the sequences of the investigator's forward and reverse primer? Enter them 5' to 3'. forward primer: reverse primer:
- PAYS- DASO บบบ 1 UUC UUA UUG CUU CUC CUA CUG U '} GUU GUC GUA GUG PHE LEU AUU AUC AUA AUG MET or START LEU ILE VAL UCU UCC UCA UCG CCU CCC CCA CCG ACU ACC ACA ACG GCU GCC GCA GCG Second base SER PRO THR ALA UAC} TYR UAA UAG } STOP CAU CAC CAA CAG} AAU AAC AAA AAG J HIS GLN ASN LYS GAU ASP GAC GAG GLU UGU CYS UGC UGA} STOP UGG TRP CGU CGC CGA CGG AGU AGC AGA AGG G GGU GGC GGA GGG ARG SER ARG GLY SCAR SCAG АSecond letter C UUU UGU UGC. UUC Phe UUA UUG UCU UCC UCA UCGJ UAU1 UAC. Ser UAA Stop UGA Stop A Tyr Leu UAG Stop UGG Trp CAUTHIS CACS CAA GIn CAG CGU CGC Arg CCU CUU CỤC CỦA CUG J CC Leu Pro ССА CGA CCGJ CGGJ AGU Ser ACU АСС ACA AUU AAU Asn AAC AAA AUC le AGA TArg Thr AUA AUG Met ACG AAGLYS AGG GAU GUU) GUC GUA GUG GCU) GCC GCA GACASP GAA AGlu GAGS GGU GGC Gly Val Ala GGA GGG GCGJ Given the following mRNA sequence: 5' GCCCAUGUGGCGCGAGUGAUUUAA 3' Third letter UCAC UCAG UCAG UCAG 등 | First letterOHHH HHHH H H H HH -с-с-с-с-с-с-с-с-с-с-с-с-с HH H H H H H H H HHH H H H H H H H HHH H-C-O C-C- OH H H H H H H H H-C-0 C-C-C-C CH, H H H,CN-C-c-o-P-0-c-H CH, H H H H H H H H H c-c-c-o What do you call the structure enclosed in the rectangle? What type of chemical bond is represented by the structure with an arrow?. What structure does the bond identified in # 2 create in relation to the entire molecule shown? True or False: This molecule is non-amphipathic. (True or False) I-0-I