Given the data below, plot the titration curve of the unknown amino acid. Determine the isoelectric point and the identity of the unknown amino acid. Volume of 0.1M KOH(mL) pH 0 1.45 0.5 1.43 1 1.45 1.5 1.47 2 1.49 2.5 1.51 3 1.54 3.5 1.58 4 1.62 4.5 1.65 5 1.7 5.5 1.74 6 1.8 6.5 1.86 7 1.92 7.5 2 8 2.09 8.5 2.22 9 2.36 9.5 2.57 10 2.84 10.5 3.42 11 5.44 11.5 8.4 12 9.72 12.5 10.41 13 11.21 13.5 11.84 14 12.19 14.5 12.37 15 12.51 15.5 12.63 16 12.71 16.5 12.78 17 12.83 17.5 12.89 18 12.94 18.5 12.99

For the titration of 70.0 mL of 0.400 M NH, with 0.500 M HCl at 25 °C, determine the relative pH at each of these points. Determine the relative pH before the addition of any HCl. pH > 7 O pH 7 Determine the relative pH after 71.0 mL HCl has been added. O pH = 7 pH > 7 O pH < 7

You made a pH 9.0 buffer solution at 25° C by mixing NaOH and histidine (HisH) to give a solution that is 0.200 M in total concentration of histidine. Deprotonation constants and enthalpies (heats) of deprotonation at 25°C Compound Deprotonation Histidine Water ▼ +H3NRH+COOH+H₂NRH COO + H+ +H3NRH+COOH3NRCOO¯ + H+ +H3NRCOO →H₂NRCOO + H H₂O-OH + H¹ Part O [Na] = Calculate the concentration of Na+ at 45 °C. Express your answer using two significant figures and include the appropriate units. Submit [H+] = 0 Ti μA Value Part N Request Answer μA Calculate the concentration of H+ at 45 °C. Express your answer using two significant figures and include the appropriate units. Value Submit Request Answer μA [OH-] = Value Submit Request Answer pK A, H° (kJ mol ¹) 1.80 6.04 9.33 14.00 Units 4 Units Calculate the concentration of OH at 45 °C. Express your answer using two significant figures and include the appropriate units. Units 29.9 46.6 55.82 ? ? ?

Can you help me find both equivalence points and pKa1 and pKa2 so I can label it on my graph?