Given the calibration curve below, what is the limit of linearity? Absorbance 2000 1800 1600 1400 1200 1000 800 600 400 200 0 (0.2 μg/ml Ο 0.4 μg/ml 0 0.2 Non-linear regresssion 0.4 0.6 0.8 R²=0.9966 1 1.2 Concentration µg/μl 1.4 1.6

How do I find the theoretical molarity?

Chemistry

Suppose that a solution has an absorbance of 0.250 at a wavelength of 450 nm.  If the concentration of the solution is 22 μM, what is the value of the molar absorptivity?  The data were taken with a standard 1-cm cuvette. molar absorptivity in (L cm^−1 mol^-1):

A medical lab technician creates a standard solutions of percent hemoglobin in whole blood. The technician measures the absorbance of each solution at 520 nm and creates a standard curve of the absorbance versus percent hemoglobin in whole blood and finds the linear equation below, with an R2 of 0.9985. y = 0.0523 x + 0.072 A patient sample has an absorbance of 0.162. Using the standard curve data, calculate the percent hemoglobin in the patient sample

What is the molar extinction coefficient at 625 nm for this copper (II) acetate solution? How was it determined? Standard Solution Concentration (M) Absorbance 1 0.025 0.049 2 0.050 0.153 3 0.100 0.242 4 0.200 0.499 5 0.250 0.694

After creating her standard curve for absorption versus the concentration (M) of FD&C Red 40, a student found that her best fit linear line for FD&C Red 40 was y = 2,962x + 0.005. Her Kool-Aid sample had an absorbance of 0.685. If 0.543 grams of Kool-Aid powder was used to prepare an 8-fl oz cup of her assigned flavor, what is the percent by mass of FD&C Red 40 in her 8-fl oz cup?

can you explain what is happening in the graph attached? Figure 1: p-nitrophenol Absorbance Standard Curve

Hh.130.

Test Tube Number Cobalt Chloride Concentration    (mol/mL) Absorbance at 510 nm       1 0.000 0.000 2 0.009 0.042 3 0.018 0.92 4 0.027 0.139 5 6 0.036 0.045 0.190 0.236   7   (Unknown)   0.151     1. what is the concentration of unknown cobalt chloride solution? 2. why is the absorbance at the absorption peak ?