Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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- In 1950, Edwin Chargaff discovered that the ratios of A:T bases, and of G:C bases, in DNA is always 1:1. This discovery became known as Chargaff's rules and was important in helping determine the structure of DNA. The percentages of adenine (A), cytosine (C), guanine (G) and thymine (T) bases in DNA from three species are shown below. Select the best answer for each of the questions. Q1: Does the distribution of nucleotide bases in species 1 and 2 in the table follow Chargaff’s rules? Why or why not? Q2: Based on the information in the table, what is the % cytosine in species 3?arrow_forwardIf a double stranded DNA HAS 20 PERCENT OF CYTOSINE, calculate the percent of adenine of adenine in the DNA?arrow_forwardWhat is the conformation of the glycosidic bond (i.e. orientation of the nucleobase relative to the the sugar) in the B-form of DNA? syn anti alpha heteroarrow_forward
- Write the base sequence and label the 3' and 5' ends of the complementary strand for a segment of DNA with the following base sequences: 5'CGGAC3'arrow_forwardA and B (adnine, cytosine, guanine, thymine) C and D (G1, G2, G3, G4)arrow_forwardPlease explain why the order is 3–1-4-2 and not another way, thank you so much. Question is in the image.arrow_forward
- 1). What is the concentration of a solution of DNA with an OD260 of 0.3? ----I got 15 micrograms/mL (50 x 0.3) 2). How many microliters of this solution of DNA would you need if you wanted 1.5 micrograms?arrow_forwardThe figure shows that the average distance between base pairs measured parallel to the axis of a DNA molecule is 3.4 Å. The average molar mass of a pair of nucleotides is 650 g•mol-1. Estimate the length in cm of a DNA molecule of molar mass 5.1x109 g•mol-1. 4.0 cm Roughly how many base pairs are contained in this molecule? 4.0 7.1e6 X base pairs AT CG OH O-P=D0 G C TA Adenine Thymine OH CH2 N-H.......O CH3 H -N N..... G C H A Он C G G C TA AT OH CH2 Cytosine Guanine H. O.......H-N H H' H N…**H-N HLH O N-H..... H2C OH TA OH CH2 (b) The most common structure of DNA, which is a right-handed double helix. The two strands are held together by hydrogen bonds and other intermolecular forces. (a) Base-pair formation between adenine (A) and thymine (T) and between cytosine (C) and guanine (G).arrow_forwardEach of the following pairs of primers has a problem with it. Tell why the primers would not work well. (a) Forward primer 5'GCCTCCGGAGACCCATTGG 3' Reverse primer 5'TTCTAAGAAACTGTTAAGG 3' (b) Forward primer 5'GGGGCCCCTCACTCGGGGCCCC 3'Reverse primer 5'TCGGCGGCCGTGGCCGAGGCAG 3' (c) Forward primer 5'TCGAATTGCCAATGAAGGTCCG 3'Reverse primer 5'CGGACCTTCATTGGCAATTCGA 3'arrow_forward
- For the following sequence please design an 18 base pair REVERSE primer. ATGGCTGATAAGATAGAGAGGCATACTTTCAAGGTCTTCAATCAAGATTTCGAAAAAGAGCTGGAGTTTGGATTAGATAGAAAATATTTTTAGarrow_forwardIn Figure 1-8b, can you tell if the number of hydrogenbonds between adenine and thymine is the same as thatbetween cytosine and guanine? Do you think that aDNA molecule with a high content of A + T would bemore stable than one with high content of G + C?arrow_forwardAlthough A-form, B-form, and Z-form DNA are all left-handed, they exhibit different physical properties due to their different numbers of bases per turn. True Falsearrow_forward
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