Finally, draw the standard curve for BSA. Reagents Blank Tube Tube Tube 2 3 Tube Tube Tube Tube 5 4 7 Stock solution of BSA= 2 mg/ml Distilled water Final concentration | (mg/ml) The 0.5 1.5 2.5 3.5 4.5 4 3.5 3 2.5 1.5 0.2 0.4 0.6 0.8 1.0 1.2 1.4 absorbance 0.086 0.422 0.665 0.727 0.858 1.2 at 280 nm Draw the standard curve with line of best fit. 3. 2. 2. 1, 5,

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Q2) Draw the standard curve for bovine serum albumin protein (BSA):
First, prepare the following solutions:
1. Stock solution of BSA with 2 mg/ml concentration.
2.7 dilutions of BSA as shown in the table.
Second, measure the absorbance for each tube at 280 nm.
Finally, draw the standard curve for BSA.
Reagents
Blank Tube Tube
2
3
Tube
Tube
Tube Tube Tube
1
4
5
6
7
Stock
solution of
BSA= 2
mg/ml
Distilled
water
Final
0.5
1
1.5
2.5
3
3.5
4.5
3.5
3
2.5
1.5
concentration
(mg/ml)
The
0.2
0.4
0.6
0.8
1.0
1.2
1.4
absorbance
0.086 0.422 0.665 0.727 0.858
1
1.2
at 280 nm
Draw the standard curve with line of best fit.
2.
2.
4.
Transcribed Image Text:Q2) Draw the standard curve for bovine serum albumin protein (BSA): First, prepare the following solutions: 1. Stock solution of BSA with 2 mg/ml concentration. 2.7 dilutions of BSA as shown in the table. Second, measure the absorbance for each tube at 280 nm. Finally, draw the standard curve for BSA. Reagents Blank Tube Tube 2 3 Tube Tube Tube Tube Tube 1 4 5 6 7 Stock solution of BSA= 2 mg/ml Distilled water Final 0.5 1 1.5 2.5 3 3.5 4.5 3.5 3 2.5 1.5 concentration (mg/ml) The 0.2 0.4 0.6 0.8 1.0 1.2 1.4 absorbance 0.086 0.422 0.665 0.727 0.858 1 1.2 at 280 nm Draw the standard curve with line of best fit. 2. 2. 4.
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