In proteomics, peptidomics (and the other -omics techniques), hyphenated MS techniques are used, and coupled with databases. (1) how are GC-MS, LC-MS, and MS-MS or MS" techniques different from each other?

Past paper question

A sample of chromatin was partially digested by the enzyme staphy- lococcal nuclease. The DNA fragments from this digestion were purified and resolved on a polyacrylamide gel. A set of DNA restric- tion fragments was used as markers. Distances of migration are given below. From these data, estimate the nucleosome repeat dis- tance in the chromatin. Marker DNA Fragment Chromatin DNA Fragment Size (bp) d (cm) d (cm) 94 40 30.5 145 34.2 19.2 263 25.2 14.4 498 16.7 11.5 794 11.5

Prepare a report (formatting specifications below) outlining the 2020 COVID-19 drug authorization process a Canadian manufacturer of COVID-19 drugs and vaccines could use, as well as the necessary regulatory submission to maintain market authorization. The report should include, but is not limited to the following elements: Purpose/objective/overview of the temporary (interim) authorization pathway. Description of: key elements that were introduced in the temporary authorization pathway to increase authorization efficiency (describe at least 3) the regulatory submission requirements the same manufacturer must fulfill to maintain market authorization following expiration of the interim authorization. Detailed critical analysis of 2 new elements introduced in the temporary authorization pathway. You may select any 2 new elements found in the temporary authorization pathway. Your analysis should indicate the advantages of disadvantages of these two components. The critical analysis should…

In order to improve the peptide separation by using a HPLC system, trifluoroacetic acid acts as mobile-phase modifier was added during the preparation of mobile phase. The preparation was performed by a postgraduate student as following:“2.851 g trifluoroacetic acid (MW: 114.02 g/mol) was made up to 500 cm3 in a graduated flask. To this solution, 50 cm3 of ethanol was added, and after mixing the mobile phase was placed in the solvent reservoir and pumping was commenced at 1.5 cm3 min-1.”Based on the given preparation procedure, identify THREE mistakes that were made.

Suh and Savizky studied the thermodynamics of human a - lactalbumin (HLA) unfolding in the absence (apo) or presence of various metal ions (Suh, J. J. and Savizky, R. M., Int. J. Biophys., 2011, 1(1), 1-6). You may assume that the protein starts out in the folded state and becomes denatured when it unfolds. According to the data below, which metal ion results in the greatest enthalpic stabilization of the folded state? AS (kJ/mol*K) 0.61 ± 0.07 0.55 ± 0.02 AH (kJ/mol) Experiment Аро-HLA MĘC1; + apo-HLA ZnCl, + apo-HLA CdCl, + apo-HLA CoCh + apo-HLA MnCl, + apo-HLA SICI, + apo-HLA CaCl + apo-HLA - apo-HLA KC1+ apo-HLA AG (KJ/mol) 184.92 ± 20.78 -5.29 ± 0.81 4.10 ± 0.17 3.62 0.13 174.05 ± 5.72 121.84 ± 6.02 0.38 ± 0.02 181.79 ± 11.56 6.06 = 0.46 0.57 ±0.04 184.69 ± 10.47 10.68 ± 0.44 0.56 ± 0.03 238.65 ± 15.22 14.10 ± 0.88 0.72 ± 0.05 15.08 ± 0.91 19.91 ± 1.33 223.41 ± 10.61 0.67 +0.03 232.16 ± 14.87 0.68 0.04 NaCl+a 162.17 ± 6.27 -4.19 + 0.18 0.54 + 0.02 154.02 ± 8.73 -3.58 ± 0.24 0.51…

MindTap - Cengage Learnir X G To analyze the reaction, firs x eploymentid=5735112480241329813180832311&elSBN=9781305862883&id=1774598910&snapshot!... Bb DO: MLM HW 2.5- Blackbox Submit Answer References Use the References to access important values if needed for this question. A chunk of iron weighing 18.1 grams and originally at 97.77 °C is dropped into an insulated cup containing 80.4 grams of water at 23.75 °C. Assuming that all of the heat is transferred to the water, the final temperature of the water is mine Csp or T Final: Thermal Equilibrium in Metal + Water: This is group attempt 1 of 10 MacBook Air Autosaved at 9:15 PM Bb My Blackbo. °C. Q 1

0.9 The enzyme from the previous problem was further purified from the supernatant, after which a (Bradford) protein assay using coomassie blue was performed. The following standard curve was established using known concentrations of the enzyme. 595 0.8 0.7 0.6 0.5 80.4 0.3 0.2 0.1 y=0.5617x+0.0119 R²=0.9878 0 0.5 Protein Concentration (mg/mL) A. At what wavelength is the sample measured at? 1.5 B. Write out the equation relating OD595 and protein concentration. C. After purification, the protein sample was diluted 10-fold and then reacted with Coomassie Blue that resulted in an OD595 of 0.7. What was the concentration of protein in the supernatant? D. The purified protein was concentrated to a final volume of 1 L. What was the final amount of purified protein recovered? 200 g of the hulls initially at 25°C was placed into a perfectly insulated calorimeter with 500 g of isopropyl alcohol (IPA) at 5°C without stirring. At equilibrium, the temperature of the IPA was found to be 13.7°C. =…