Explain the role/importance of the localization of GTPase-activating protein (Ran-GAP) in BOTH nuclear export and import. What would occur if there was a loss offunction mutation in Ran-GAP? What would occur if Ran-GAP was localized in thenucleus?

The Ran protein is a member of the Ras superfamily of small GTPases. Ran regulates nucleocytoplasmic…

Answered: Explain the role/importance of the…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Rather surprisingly, some cellular components that we know best from our study of nuclear protein traffic also appear to be involved in the process of spindle assembly. These include Ran GEF (which, as you may recall, is bound to chromatin – an association that apparently continues even when the nuclear envelope breaks down at the onset of mitosis) and importin-β. Now think back to the process of nuclear protein import. To which form of Ran (Ran-GDP or Ran-GTP) does importin-β bind and what is the significance of that interaction with respect to the efficiency of delivery of import “cargo” to the nucleus? To which form of Ran (Ran-GDP or Ran-GTP) do exportins bind and what is the significance of that interaction with respect to the efficiency of delivery of export “cargo” to the cytoplasm?
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Explain the role/importance of the localization of GTPase-activating protein (Ran- GAP) in BOTH nuclear export and import. What would occur if there was a loss of function mutation in Ran-GAP? What would occur if Ran-GAP was localized in the nucleus?