Explain the process of rDNA technology

Q: CRISPR is a tool used by bacteria to fight virus attacks. In your own words, describe how CRISPR…

A: CRISPR, used as a defense tool by bacteria to fight virus attacks.

Q: Explain why exome sequencing can be almost as valuable as genome sequencing.

A: Whole genome sequencing refers to technique which used to analyze complete genome of an organism at…

Q: Explain the Importance of protein separation in biotechnology

A: Introduction: The separation of proteins is an important step in biotechnology. It plays an…

Q: Explain the role of restriction endonucleases in the process of genetic engineering.

A: Genetic engineering is a method adopted by various researchers to alter the genetic makeup of an…

Q: Explain how recombinant DNA technology uses restriction enzymes (restriction endonucleases). Explain…

A: Recombinant DNA technology is the combining of the DNA molecules from two different species. The…

Q: Outline and discuss the steps in DNA cloning

A: Gene cloning, also known as molecular cloning, is the method of isolating a DNA sequence of interest…

Q: Contrast how gene cloning is different betweenrecombinant technology and PCR.

A: Gene cloning: It is also known as molecular cloning, it refers to the process of isolation of a DNA…

Q: Discuss the concept of the use of DNA in genome identification.

A: DNA (Deoxyribo nucleic acid) is a double stranded genetic element in all prokaryotic and eukaryotic…

Q: Compare the information that can be obtained fromgenomic and cDNA libraries

A: Deoxyribonucleic acid (DNA) libraries are constructed by scientists using genetic engineering…

Q: Which of the following scenarios could use Next-Gen sequencing to achieve its purpose? Detect…

A: Genomic research has been transformed by next-generation sequencing (NGS), a massively parallel or…

Q: Briefl y summarize the steps involved in DNA sequencing and describe its applications.

A: DNA Sequencing is the process or method which determines the order of the four nucleotides bases…

Q: To clone a bacterial lipase gene into the cloning vector pET28a, you know the full sequence of the…

A: For the ease of manipulation, vectors must be relatively small molecules. They must be capable of…

Q: Describe the function of restriction enzymes and ligase in genetic recombination technology.

A: The branch of biology that deals with forming a recombinant DNA by joining the DNA segments from two…

Q: Briefly explain about computer-automated high-throughput DNA sequencing ?

A: DNA sequencing means identifying the sequence of DNA by scientific means. High-throughput DNA…

Q: Explain the purpose of the antibiotic resistance gene in this experiment. Why is this genetic trait…

A: Bacteria are microorganism that most commonly occur in the soil, air, water and in adverse…

Q: Give the meanings of the following terms: genomics, functionalgenomics, and proteomics.

A: Genetics is defined as the study of genes. Genes are the functional and structural unit of life.…

Q: Explain how CRISPR was discovered and developed into the tool available today.

A: CRISPR stands for clustered regularly interspaced short palindromic repeats. These were naturally…

Q: Outline the steps by which the polymerase chainreaction (PCR) amplifies a specific region of a…

A: Polymerase chain reaction or PCR technique is used to amplify or make multiple copies of a specific…

Q: Biotechnology is a constantly developing field with many exciting applications. It focuses on the…

A: Gene transfer is the method of inserting genetic material (DNA or a gene of interest) into cells…

Q: Describe CRISPR as a genetic tool with its functions .

A: CRISPR ( Clustered Regularly Interspersed Short Palindromic Repeats ) is a segment of prokaryotic…

Q: Briefly explain how the polymerase chain reaction is used to amplify a specific DNA sequence. What…

A: PCR which stands for polymerization chain reaction is a rapid and extremely sensitive analytical…

Q: What is gene sequencing used for?

A: DNA sequencing is the process of determining the sequence of nucleotide bases that make up a DNA.…

Q: Describe the role of Genetic engineering in biotechnology.

A: Biotechnology refers to the field that uses technology in biological systems. The term…

Q: Explain the Importance of protein separation in biotechnology

A: The separation of protein is an important step in biotechnology. It plays the vital role in various…

Q: Outline the steps in automated DNA sequencing via the dideoxy method.

A: DNA sequencing:   It is a method to find a sequence of nucleotides by using the DNA sequencing…

Q: Define PCR. Write down different types of PCR in detail with their applications. Give the answer…

A: Polymerase chain reaction (PCR) was invented by Dr.Kary Mullis in the year 1983. It is called…

Q: Distinguish between the general & technical uses of the term “biotechnology”. What is Recombinant…

A: Biotechnology has improved our daily lives from GMOs to various medicines biotechnology has enhanced…

Q: using the process flow of PCR describe the principle associated with the PCR technique.

A: Introduction :  Polymerase Chain Reaction or PCR is a widely employed technique in molecular biology…

Q: What are the overall benefits/consequences of the Human Genome Project and the ability to quickly…

A: DNA sequencing is the process of determining the exact order of nucleotides (A, C, G, T) in a DNA…

Q: How would you go about cloning this amplified DNA into pL4440？Using your knowledge of cloning…

A: Cloning is the process of creating genetically identical copies of a biological entity, such as a…

Q: Provide one example of a CRISPR-Cas application forbiotechnology.

A: Two crucial components make up the CRISPR-Cas9 system, which modifies DNA. These include the Cas9…

Q: Discuss the principles,uses , advantages and disadvantages of SMART sequencing.

A: Single-molecule real-time sequencing is a parallelized single-molecule DNA sequencing method.…

Q: How can site-specific recombination be used in recombinant DNA technology? Explain with an example

A: DNA manipulation is an important aspect of many biotechnology applications because it allows…