Explain the activities associated with the isolation of Bacillus megaterium organsim from mixed culture to pure culture. Include the inoculum source, incubation temperature and other information.
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Explain the activities associated with the isolation of Bacillus megaterium organsim from mixed culture to pure culture. Include the inoculum source, incubation temperature and other information.
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- Explain the activities associated with the isolation of Bacillus subtilis organsim from mixed culture to pure culture. Include the relevant information( type of inoculation, source of bacteria etc)? Also the isolation of the organism from the broth containing a fresh mixture of 2 or more bacteria was obtained after incubation of nutreint agar plate.Explain the activities associated with the isolation of Bacillus subtilis organsim from mixed culture to pure culture. Include the inoculum source, incubation temperature and other information.Solve the identity of an unknown bacterial specimen by creating a dichotomous key. Categories: Gram stain, Mannitol salt agar,MacConkey agar, Catalase, Oxidase, Casein hydrolysis, Nitrate reduction, Citrate test, Urease test, Sim Medium Streptoccocus ID: 1) Bile Esculin Test 2) Bacitracin Test 3) Blood Agar Plate NaCl Broth Staphylococcus ID: 1) Mannitol Salt Agar 2) DNA Hydrolysis 3) Coagulase Test 4) Novobiocin Test Possible Organisms Alcaligenes faecalis Enterobacter aerogenes Enterococcus faecalis Escherichia coli Proteus vulgaris Pseudomonas aeruginosa Salmonella arizoniae Staphylococcus aureus Staphylococcus epidermidis Staphylococcus saprophyticus Streptococcusbovis Streptococcus pyogenes
- discuss the clinical significance, principles, reagents (if any) and positive and negative results of the following laboratory tests for Gram positive cocci organisms: Bile esculin test 5% salt turbidity or tolerance test PYRase test Pyruvate broth testExplain the significance of the Snyder agar medium and its differential nature as a means to detect dental caries.explain how the use of an Eosin methylene blue agar plate can help determine the type of fermenters that bacteria are. explain throughly.
- Can you please discuss the principles of using Motility Test Medium for the detection of bacterial motility. Focus on the components of the medium and the purpose of each component. Note: You may refer to different culture media manuals like Difco, BBL, etc.Based on the found under #4 inoculating plates order, and the definitions above, what can you assume the order of plating cultures is based on? *1) What are all of the different colony types that can be seen on a mixed-culture streak plate? 2) What kinds of colonies can be seen when mixed cultures of E. coli, M. luteus, and Serratia marcesens? 3) What is the range of colonies that may be seen with each mixed culture?
- Below are morphological, physiological and biochemical characteristics of an unknown luminous bacterium isolated from the gills of a marine fish. Use the data below to identify the isolate. Make an identification scheme Test Unknown Isolate B 1 Luminescence Positive 2 Cell Shape Straight Rods 3 Gram Staining Negative 4 Oxygen Requirement Facultative Anaerobe 5 Motility (Hanging Drop) Motile 6 Growth at 0% NaCl Negative 7 3% NaCl Positive 8 6 % NaCl Positive 9 Accumulation of PHB Positive 10 Flagella 1 Polar 11 Oxidase Positive 12 Nitrate Reduction 13 Gelatinase Negative 14 Growth at 35OC Positive 15 Growth at 4OC Negative 16 Methyl Red Positive 17 Voges-Proskauer Positive 18 Catalase Positive 19 Starch Hydrolysis Negative 20 Lysine…Name six basic nutritional requirements supplied in all culture media.what is the physical characteristics of this streak plate? Look at a single colony on a streak plate and look for special physical characteristics such as: motility, possible presence of endospores and/or capsule, culture color?