What effect will the following factors have on a liquid chromatographic separation?

The following statements are true for any chromatographic analysis except: The analyte component interacting most strongly with the stationary phase elute faster than those with weaker interactions. On the other hand, those with least interaction with the mobile phase elute the slowest. Interactions between mobile phase-analyte - stationary phase can be both chemical and physical in nature. Chromatography is a separation technique. Electromagnetic radiation source for XPS. Gamma ray X-ray UV-Visible Infra red Radiowave The part of SEM that is responsible for the production of electrons. * Electron guns Condenser lens Secondary detector Electron beam

Brief discussion of column chromatographic separation (solvent polarity, elution ofcompounds etc.)

A 20-cm long packed column was used to perform the separation of A and B by LC. TRA=16.28 min, TR,B=17.50 min, WA=1.11 min, WB=1.19 min, tм=1.30 min. (a) Calculate the retention factor (k') for A and B. (b) Calculate the average number of theoretical plates and plate height.