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- Describe the effects of intron phase on alternative splicing with diagramWhen an intron is undergoing the first step in splicing, where is the first breakage of the phosphodiester backbone that occurs? (Explain)Suppose that a 20-bp deletion occurs in the middle of exon 2 of the gene depicted in Figure 14.12a. What will be the likely effect of this deletion in the proteins produced by alternative splicing?
- Why do you expect that intron removal wouldreduce the delay?After the intron (which is in a lariat configuration) is released during pre-mRNA splicing, a brief moment occurs before the two exons are connected to each other. Which snRNP(s) hold(s) the exons in place so they can be covalently connected to each other?How does the presence of introns aid exon shuffling?
- Describe how mature messenger ribonucleic acids (MRNAS) are post-transcriptionally processed in the nucleus before they are transported to the cytoplasm of a eukaryotic cell.Explain why histone eviction is needed for the elongation phase of transcription.Assume alternative splicing can generate all permutations and combinations. How many proteins could a gene with 3 exons and 2 introns generate?
- Suppose that a 20-bp deletion occurs in the middle of exon 2 of the gene. What will be the likely effect of this deletion in the proteins produced by alternative splicing?What is meant by the term histone code? With regard to gene regulation, what is the proposed role of the histone code?Is an entire chromosome made into an mRNA during transcription?