Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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Explain how interrupted conjugation, transformation, and
transduction can be used to map bacterial genes. How are these
methods similar and how are they different?
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- If a vector containing the desired gene X is inserted into a microorganism, for instance, A.carbornarious, and the protoplast transformation resulted in 9 gene X transformants, how to determine which transformant has the highest titer of gene X?arrow_forwardWhat is a recombinant microorganism? Discuss examples.arrow_forwardAn 9 kb circular plasmid is cut with the EcoRI restriction enzyme, and the reaction products are run on a DNA gel and stained with ethidium bromide. Bands of 1, 3 and 5 kb are seen. How many EcoRI sites are in the plasmid? Choose the one answer that is most correct. a) At least 2 b) At least 3 c) At least 1 d) None e) At least 4 f) At least 5arrow_forward
- Describe two methods for transforming a vector into bacteria.arrow_forwardBriefly explain how a gene can be isolated through positional cloning.arrow_forwardOutline a strategy for constructing a genomic DNA library more representative of the entire humangenome. You will need to consider alternative vectors and the efficiency of transformation of thebacterial cellsarrow_forward
- Assume you have successfully cloned a small (200 bp) fragment of DNA into the polylinker region of a pUC18 cloning vector. Describe the appearance of transformed colonies you would expect to see on each of the following plates: plain media, media containing ampicillin, media containing tetracycline, media containing ampicillin and X-Gal.arrow_forwardMicrobiology, Horizontal Gene Transfer Describe the role of recombination in gene transfer: which mechanisms require recombination? What happens if recombination cannot occur? What, specifically, is required for recombination to occur?arrow_forward
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