Experiment 1: Does Enzyme Concentration affect Amylase Activity? To investigate the effect of enzyme concentration on amylase activity, we mixed 6 ml of 0.5% amylose solution with 70ul of amylase enzyme solution. Each trial used a different concentration of enzyme solution. At 15 second intervals, we tested the reaction mixture for the presence of amylose starch by dropping the mixture into a well containing a drop of Lugol's iodine. We compared our results against the following: a well that contained only amylase solution and iodine, a well containing only enzyme solution and iodine, and a well containing completely digested (or broken-down) starch solution and iodine. We recorded the time of completion of the reaction for each of our trials, when no more starch was present, as the first sample that did not show a positive starch reaction.

Experiment 1: Does Enzyme Concentration affect Amylase Activity? To investigate the effect of enzyme concentration on amylase activity, we mixed 6 ml of 0.5% amylose solution with 70ul of amylase enzyme solution. Each trial used a different concentration of enzyme solution. At 15 second intervals, we tested the reaction mixture for the presence of amylose starch by dropping the mixture into a well containing a drop of Lugol's iodine. We compared our results against the following: a well that contained only amylase solution and iodine, a well containing only enzyme solution and iodine, and a well containing completely digested (or broken-down) starch solution and iodine. We recorded the time of completion of the reaction for each of our trials, when no more starch was present, as the first sample that did not show a positive starch reaction.

3-2-1 Code It

6th Edition

ISBN:9781337660549

Author:GREEN

Publisher:GREEN

Chapter17: Cpt Pathology And Laboratory

Section: Chapter Questions

Problem 15MC

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