expen using disinfectant yielded the following results: Control (no disinfectant) - 220 colonies on a Petri dish Experimental (with disinfectant) - 100 colonies on a Petri dish Is the disinfectant bactericidal or bacteriostatic? O Bacteriostatic O Bacteicidal
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- What is the unknownn organism based on this biochemical test results? Gram Stain: Gram Positive Oxidase: Positive Catalase: Positive MAC: Non Lactose Fermenter MSA: Mannitol Salt Fermenter Gelatin Hydrolysis: Negative Indole Production: Negative Motility Indole Ornithine Medium: Negative MR/VP Test: Negative Nitrate Test: Positive Simmon Citrate Test: Negative Urease Test: Positive Possible Organisms: Vibrio fischeri Enterococcus faecalis Micrococcus luteus Streptococcus pneumoniae Pseudomonas aeruginosa Proteus mirabilis Acinetobacter baumannii Staphylococcus aureus Mycoplasma pneumoniae Escherichia coli Candida albicans Salmonella enterica Listeria monocytogenesMSA (Mannitol Salt Agar) Match the description to the correct Bacterium A= growth, agar is yellow B= Growth, agar is not yellow C= no growthA new chemical disinfectant was tested for activity against Staphylococcus growth. The bacteria were incubated in bacterial growth medium + disinfectant for 24 hours, then one loopful of the mixture was transferred to fresh medium without the disinfectant (subculturing) and incubating the subculture. The initial culture and subcultures were evaluated for growth after incubation. (+ = growth; - = no growth) Dilution Initial Subculture 1:2 - - 1:4 - - 1:8 - + 1:16 + + 1:32 + + Based on these results, which of the following statements is true regarding the effectiveness of this disinfectant against this bacterium? The disinfectant is bacteriostatic at 1:4 dilution. The disinfectant is bacteriostatic at 1:2 dilution. The disinfectant is NOT bacteriostatic at any of the dilutions tested. The disinfectant is bacteriostatic at 1:8 dilution. The disinfectant is bacteriostatic at 1:16 dilution.
- A class of 15 students (8 males and 7 females) will need to culture an unknown bacteria for a specific activity where 5 nutrient agar pates per female student and 3 agar slants per males student are needed to prepare. agarplate: 25mL Agar slant: 10mL Broth tube: 8mL Nutrient Agar: Yeast extract: 2g/L peptone: 5g/L Sodium chloride: 5g/L Agar powder: 15g/LGiven the illustration and values below, determine the concentration of the original sample. Report results in CFU/mL or colony-forming unit/milliliter. Note that in order to observe the accuracy of results, culture plates with countable colonies between 25-250 CFU are considered in standard bacterial plate count.Describe characteristics of Streptococcus Agalactiae in the Agar: (How does colonies look like (color) and explain does it grow on that agar. (Don't have to write the incubation period) ~ Only describe how would it look like on the Agar: Blood Agar (Aerobic) MacConkey EMB PEA Mannitol Salt Agar Chocolate Agar Nutrient Agar
- The figure attached shows the colonies on Eosin Methylene Blue Agar (EMBA) plate. Make a description on the picture.If 5% w/v concentration of a bactericide of dilution coefficient of 3 takes 25 minutes to effect killing, what will be the time of kill when it is diluted 8 times A sanitizer with dilution coefficient of 0.5 takes 3 minutes to effect killing. If it takes 18 minutes to kill when it is diluted 5times, what is the initial concentration? An unknown concentration of ‘LD’ disinfectant killed 106 viable cells of Sporotrichum schenkii within a certain time. When the concentration was increased 2-fold the extinction time decreased a 103-fold. Determine the concentration exponent of this disinfectantA culture of E. coli has a concentration of 5 x 108cells/mL. How many times do you have todilute the culture so that when you spread 0.1mL on an agar plate you will have 250 colonies?Hint: you need to find dtotal and then convert it into a DF.
- Below are morphological, physiological and biochemical characteristics of an unknown luminous bacterium isolated from the gills of a marine fish. Use the data below to identify the isolate. Make an identification scheme Test Unknown Isolate B 1 Luminescence Positive 2 Cell Shape Straight Rods 3 Gram Staining Negative 4 Oxygen Requirement Facultative Anaerobe 5 Motility (Hanging Drop) Motile 6 Growth at 0% NaCl Negative 7 3% NaCl Positive 8 6 % NaCl Positive 9 Accumulation of PHB Positive 10 Flagella 1 Polar 11 Oxidase Positive 12 Nitrate Reduction 13 Gelatinase Negative 14 Growth at 35OC Positive 15 Growth at 4OC Negative 16 Methyl Red Positive 17 Voges-Proskauer Positive 18 Catalase Positive 19 Starch Hydrolysis Negative 20 Lysine…What is the unknownn organism based on this biochemical test results? Gram Negative Oxidase Positive Catalase Positive MAC: Lactose Fermenter MSA: Mannitol Fermenter Gelatin Hydrolysis: Negative Indole Production: Negative Motility Indole Ornithine Medium: Negative MR/VP Test: Negative Nitrate Test: Negative Simmon Citrate Test: Positive Urease Test: Positive Possible Organisms: Vibrio fischeri Enterococcus faecalis Micrococcus luteus Streptococcus pneumoniae Pseudomonas aeruginosa Proteus mirabilis Acinetobacter baumannii Staphylococcus aureus Mycoplasma pneumoniae Escherichia coli Candida albicans Salmonella enterica Listeria monocytogenesYou are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial dilutions of the soil in sterile water, 100 uL volumes are taken from each dilution for preparation of pour plates. Following incubation, each half of the 10-8 plate has 46 colonies.a) What was the dilution factor?b) How many bacteria were present in the soil?2. Staphylococcus aureus divides every 20 minutes. A culture begins with 10 bacterial cells.a) After 5 hours, how many generations have occurredb) After 5 hours, how many bacteria are present?3. How many milliliters would you need to prepare a 10-2 dilution from a 10ml starting culture?