enzymatic activity. Which of the following procedures might help you to obtain soluble, enzymatically active protein? Select all options that may be useful. Explain your reasoning. A. Make the bacteria synthesize protein A in smaller amounts. B. Dissolve the protein aggregate in urea, then dilute the solution and gradually remove the urea. C. Treat the insoluble aggregate with a protease. D. Make the bacteria overproduce chaperone proteins in addition to protein A. E. Heat the protein aggregate to denature all proteins, then cool the mixture.
enzymatic activity. Which of the following procedures might help you to obtain soluble, enzymatically active protein? Select all options that may be useful. Explain your reasoning. A. Make the bacteria synthesize protein A in smaller amounts. B. Dissolve the protein aggregate in urea, then dilute the solution and gradually remove the urea. C. Treat the insoluble aggregate with a protease. D. Make the bacteria overproduce chaperone proteins in addition to protein A. E. Heat the protein aggregate to denature all proteins, then cool the mixture.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:You wish to produce a human enzyme, protein A, by
introducing its gene into bacteria. The genetically
engineered bacteria make large amounts of protein A,
but it is in the form of an insoluble aggregate with no
enzymatic activity. Which of the following procedures
might help you to obtain soluble, enzymatically active
protein? Select all options that may be useful. Explain
your reasoning.
A. Make the bacteria synthesize protein A in smaller
amounts.
B. Dissolve the protein aggregate in urea, then dilute
the solution and gradually remove the urea. C. Treat the
insoluble aggregate with a protease.
D. Make the bacteria overproduce chaperone proteins
in addition to protein A.
E. Heat the protein aggregate to denature all proteins,
then cool the mixture.
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