"DNA polymerase I serves a secondary function in vivo,now believed to be critical to the maintenance of fidelityof DNA synthesis" Explain this statement ?
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A: DNA microarray analysis is a technique use to identify the expression of genes present in a DNA…
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A: Introduction DNA pol III is the primary enzyme which leads the DNA replication system. It is a…
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Q: ndicate whether each of the following statements are true of depurination, deamination or pyrimidine…
A: DNA is a long polymer of nucleotide. A nucleotide is made ip of sugar, nitrogenous bases, and…
Q: Just question 28
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A: DNA polymerase is an enzyme, which helps to synthesize new complementary strands with the…
Q: Reiji and Tuneko Okazaki conducted a now classic experiment in1968 in which they discovered a…
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Q: If DNA polymerase requires a perfectly pairedprimer in order to add the next nucleotide, how is it…
A: DNA polymerases are enzymes that manufacture DNA molecules by assembling nucleotides, which are the…
Q: Polymerases usually add only about 10 nucleotides toa DNA strand before dissociating. However,…
A: Nucleotides are the molecules composed of a sugar moiety, phosphate group, and a nitrogenous base.…
Q: Contrast the various types of DNA repair mechanisms known to counteract the effects of UV radiation.…
A: A variation is an adjustment in a DNA grouping. Changes can result from DNA duplicating botches made…
Q: Polymerases usually add only about 10 nucleotides to a DNA strand before dissociating. However,…
A: The DNA replication is carried out by an enzyme called as DNA polymerase enzyme that can only add…
Q: DNA ligase has the ability to relax supercoiled circular DNA in the presence of AMP but not in its…
A: The relaxed DNA is a type of conformation, which moves slowly in the gel of electrophoresis. The…
Q: Close contact. Examination of the structure of DNA polymerases bound to nucleotide analogs reveals…
A: DNA polymerases are the enzyme that replicates DNA in cells. DNA polymerase can not create new…
Q: What basal trasncription factors can only bind to the dna within the pre-replication complex after…
A: Basal transcription factors are also known as general transcription factors. These bind to the…
Q: Describe an experimental approach to determining the processivity of a DNA polymerase (i.e., the…
A: Processivity is the average number of bases a polymerase enzyme will extend and incorporate base…
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Q: For each DNA repair process in column I, list all characteristics fromcolumn II that correctly…
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Q: "Polymerase binding is never very efficient unless CAP is alsopresent to facilitate the process".…
A: A functional unit of DNA comprising a cluster of genes controlled by a single promoter is called an…
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Q: Explain why Mismatch Repair of absolutely requires semi-conservative DNA replication during S phase
A: * DNA mismatch repair is used for recognizing and repairing the insertions and deletions and…
Q: PRDM9 recruits SPO11, a topoisomerase type II like protein for inducing DNA double strand breaks.…
A: *PRDM9 is a DNA binding and zinc finger protein that trimethylates histone H3K4 modification…
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Q: PRDM9 recruits SPO11, a topoisomerase type II like protein, to induce DNA double strand breaks.…
A: Introduction PRDM9 is a DNA binding and zinc finger protein that trimethylates histone H3K4…
Q: DNA polymerases are capable of editing and error correction, meaning it is able to edit and correct…
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A: The process by which DNA makes its own copy is known as DNA replication. It start from a point known…
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Q: In eukaryotes, what is meant by the term DNA replication licensing? How does the process occur?
A: BASIC INFORMATION DNA Replication It is the process in which there is production of the replicas…
Q: Richard Boyce and Paul Howard-Flanders conducted an experimentthat provided biochemical evidence…
A: DNA repair mechanisms are operative to prevent possible mutations in the DNA.
Q: DNA polymerase III in bacteria is responsible for initiating DNA replication by adding nucleotides O…
A: Introduction:- Replication is a process by which a cell duplicates it's genetic material. There are…
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A: ATP-dependent chromatin remodeling complexes i.e. SWI /SNF complex are specific machinery that can…
Q: A certain mutant DNA polymerase is error-prone, tending to incorporate C opposite a template A. When…
A: Mutations in the DNA polymerase would affect the process of replication. It is studied under the…
Q: During DNA replication, the function of RNA primers is to O serve as a binding site for DNA ligase…
A: Introduction : Primer is a short sequence that is synthesised by primase (a type of RNA polymerase)…
Q: Explain why the active site of poly(A) polymerase is much narrower than that of DNA and RNA…
A: Poly (A) polymerase is the enzyme that is an important part of the polyadenylation machinery. It is…
Q: Is RNA polymerase saturated with substrates in vivo? Describe experiments that might indicate…
A: RNA polymerase is an enzyme involved in the process of transcription and is responsible for binding…
Q: What are the requirements for in vitro synthesis of DNA under thedirection of DNA polymerase I?
A: The formation of deoxyribonucleic acid molecules, either naturally or artificially, is known as DNA…
"DNA polymerase I serves a secondary function in vivo,
now believed to be critical to the maintenance of fidelity
of DNA synthesis" Explain this statement ?
Step by step
Solved in 2 steps
- "Polymerase binding is never very efficient unless CAP is alsopresent to facilitate the process". Explain this ?Polymerases usually add only about 10 nucleotides toa DNA strand before dissociating. However, during replication, DNA pol III can add tens of thousands ofnucleotides at a moving fork. How is this additionaccomplished?Explain why base excision repair, nucleotide excision repair, and mismatch repair—which all require nucleases to excise damaged DNA—require DNA ligase.
- In a bacterial culture in which all cells are unable to synthesizeleucine (leu-), a potent mutagen is added, and the cells areallowed to undergo one round of replication. At that point, samplesare taken, a series of dilutions are made, and the cells areplated on either minimal medium or minimal medium containingleucine. The first culture condition (minimal medium) allowsthe growth of only leu+ cells, while the second culture condition(minimal medium with leucine added) allows growth of all cells.The results of the experiment are as follows: Culture Condition Dilution ColoniesMinimal medium 10-1 18Minimal medium + leucine 10-7 6What is the rate of mutation at the locus associated with leucinebiosynthesis?What is meant by non-classical DNA-dependent DNA polymerases? What roles do they play in our cells?PRDM9 recruits SPO11, a topoisomerase type II like protein for inducing DNA double strand breaks. Illustrate how SPO11 releases SPO11-linked oligonucleotides. r
- Point mutations in multiple tumor suppressor proteins have been linked to cancer. For example changes in the gene for adenomatous-polyposis-coli protein (APC gene) may result in colorectal cancer. Consider the following DNA sense strand. 3'-TAC CGG TTG TGA AGC TGA ATC-5' (i) (ii) (iii) (iv) Derive the mRNA molecule from the given DNA strand sequence above, paying attention to the polarity of the molecule. Write down the polypeptide chain sequence arising from the mRNA molecule of the question above, using the table of the genetic code (Table Q1 overleaf) and indicate the C- and the N-terminus of the peptide chain. Point mutations of a cytosine (C) often lead to the dysfunction of the APC protein. Write down all possible polypeptide chains that can result from all possible DNA mutations of cytosines, disregarding a mutation in the MET/START and STOP codons. I Specify which of the point mutations identified in (d) are redundant?In Figure 12-26, provide a biochemical mechanism forwhy HP-1 can bind to the DNA only on the left side of thebarrier insulator. Similarly, why can HMTase bind onlyto the DNA on the left of the barrier insulator?How was it demonstrated that DNA synthesis occurs underthe direction of DNA polymerase III and not polymeraseI?
- . The double-stranded circular DNA molecule thatforms the genome of the SV40 tumor virus can be denatured into single-stranded DNA molecules. Becausethe base composition of the two strands differs, thestrands can be separated on the basis of their densityinto two strands designated W(atson) and C(rick). When each of the purified preparations of the single strands was mixed with mRNA from cells infectedwith the virus, hybrids were formed between the RNAand DNA. Closer analysis of these hybridizationsshowed that RNAs that hybridized with the W preparation were different from RNAs that hybridized withthe C preparation. What does this tell you about thetranscription templates for the different classes ofRNAs?PRDM9 recruits SPO11, a topoisomerase type II like protein, to induce DNA double strand breaks. Illustrate Illustrate and explain and explain how SPO11 releases SPO11-linked oligonucleotides.During high stress environments, it has been found that some bacteria activate a genetic mechanism that allows them to incorporate more mutations into the DNA during replication. Would the following two enzymes be impacted by such a mechanism? (i)DNA polymerase IIIii) Helicase