DNA MELTING Two antiparallel single strands form a DNA duplex according to the following model: SA SB SASH a) You have carried out melting studies at various concentrations for an unknown DNA. Here is the fitted data (1) (ii) (iii) (iv) " y=0.0000271x + 0.00282. Based on the best-fit equation above, find the unknown sequence from the options below given the free energy data at 37°C. Show your work AGGCGATCGG and its complement AGCAATGGCC and its complement GGAGAACTTA and its complement GGCCAAGGCC and its complement AºG - 9.50 kcal/mol 35.97 kJ/mol 30.55 kJ/mol -10.20 kcal/mol - b) Thermodynamically speaking, AS and AH can both drive processes. What happens to these parameters as double stranded DNA is melted? Explain.
DNA MELTING Two antiparallel single strands form a DNA duplex according to the following model: SA SB SASH a) You have carried out melting studies at various concentrations for an unknown DNA. Here is the fitted data (1) (ii) (iii) (iv) " y=0.0000271x + 0.00282. Based on the best-fit equation above, find the unknown sequence from the options below given the free energy data at 37°C. Show your work AGGCGATCGG and its complement AGCAATGGCC and its complement GGAGAACTTA and its complement GGCCAAGGCC and its complement AºG - 9.50 kcal/mol 35.97 kJ/mol 30.55 kJ/mol -10.20 kcal/mol - b) Thermodynamically speaking, AS and AH can both drive processes. What happens to these parameters as double stranded DNA is melted? Explain.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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Transcribed Image Text:DNA MELTING
Two antiparallel single strands form a DNA duplex according to the following model:
S.
S, S,
a) You have carried out melting studies at various concentrations for an unknown DNA. Here is the
fitted data
y = - 0.0000271 x + 0.00282.
Based on the best-fit equation above, find the unknown sequence from the options below given the
free energy data at 37°C. Show your work
(i)
(ii)
(iii)
(iv)
AGGCGATCGG and its complement
AGCAATGGCC and its complement
GGAGAACTTA and its complement
GGCCAAGGCC and its complement
A°G
- 9.50 kcal/mol
35.97 kJ/mol
30.55 kJ/mol
-10.20 kcal/mol
b)
these parameters as double stranded DNA is melted? Explain.
Thermodynamically speaking, AS° and AH° can both drive processes. What happens to
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