Discuss the two main limitation of plant hybridization programm?

Why is translocation important to plants?

In an analysis of five rII mutants, complementation testing yielded the following results:

Explain the experimental advantage of genetic mapping?

Briefly Explain different applications of western blotting. Please explain at your own words.

6). To investigate the genetic control of locomotive behavior in the diploid nematode, Caenorhabditis elegans, eight "wiggly" mutants were isolated, which wiggle in an uncoordinated way rather than smoothly gliding. These eight mutants were crossed together in all possible pairwise combinations, yielding the results summarized in the table above (+ means that all the progeny were wild type, and m means that all the progeny showed the wiggly mutant phenotype). Organize the eight mutations into con lementation groups, each consisting of a group of mutations affecting the same gene. 1 2 3 4 5 6 7 8 1 E N+E 2 3+1 m + m 4 5 + m m E+ + + EE 6 ++E E+ +EE m m + + + + + + + + + m 7 8 ++4 ∞+E +E m m ++ E

What is in situ hybridization used for and how  does In Situ Hybridization detect?

Examine the variegated leaf shown in Figure Q14–3.Yellow patches surrounded by green are common, butthere are no green patches surrounded by yellow. Proposean explanation for this phenomenon.

What is inverse PCR? How are we going to use inverse PCR to help figure out the molecular location of the EP element on Drosophila melanogaster flies?

You are a developmental geneticist studying flowering time variation in Arabidopsis. You perform a mutagenesis screen to identify mutants in the photoperiod pathway. Given what you know about photoperiodism in Arabidopsis, what phenotype are you looking for and under what photoperiodic conditions would you perform the experiment? delayed flowering in long days delayed flowering in short days same flowering in short days early flowering in short days same flowering in long days early flowering in long days